<noautolink> ---+++ Sterile Saline *Purpose: Used make dilutions of viable cells for plating or transfer to new media.* This saline concentration of 0.85% w/v (145 mM) is suitable for diluting _E. coli_ before transferring to new media or plating on agar. | *1L* | *5L* | *Component* | | 8.5 g | 42.5 g | NaCl | Add H<sub>2</sub>O to final volume. Autoclave before use. Prepare large batches in 6 L flask and then aliquot out into bottles. It is not important to measure out exact volumes when dividing a large batch among bottles.
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Topic revision: r3 - 2011-07-29 - CraigBarnhart