<noautolink> ---+++ S2 Used for Acinetobacter | *1L* | *5L* | *Component* | | 1.5g | 7.5 g | KH<sub>2</sub>PO<sub>4</sub>| | 25.5g | 127.5 g | Na<sub>2</sub>HPO<sub>4</sub> 7H<sub>2</sub>O| | 0.1g | 0.5 g | MgSO<sub>4</sub>| | 2g | 10 g | NH<sub>4</sub>Cl| | 0.01g | 0.05 g | CaCl<sub>2</sub>| | 0.0005g | 0.0025 g | FeSO<sub>4</sub> 7H<sub>2</sub>O| To each 1L, add 5ml of 85-90% lactic acid. Bring final pH to 6.65. Calcium Chloride may be better added as 1ml of 1% solution, or 90ul of 1M solution. Ferrous Sulfate may be better added as 0.5ml of a 0.1% solution. It might be best to autoclave the calcium chloride separate from the sulfates, as there seems to be a risk of calcium sulfate precipitate otherwise. | *Final composition* || | 190.2 mM | Sodium (Na<sup>+</sup>) | | 11.0 mM | Potassium (K<sup>+</sup>) | | 37.8 mM | Ammonium (NH<sub>4</sub>) | | 0.09 mM | Calcium (Ca<sup>2+</sup>) | | 0.83 mM | Magnesium (Mg<sup>2+</sup>) | | 38.0 mM | Chloride (Cl<sup>-</sup>| | 0.87 mM | Sulfate (SO<sub>4</sub><sup>2-</sup>) | | 106.1 mM | Phosphate (PO<sub>4</sub><sup>3-</sup>) | | 50.0 mM | Lactic Acid| -- Main.AurkoDasgupta - 03 Jun 2013
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Topic revision: r4 - 2013-06-25 - AurkoDasgupta