M9 Minimal Media Plates

1L Component
6 g Sodium phosphate, Na2HPO4H2O
3 g Potassium phosphate, KH2PO4
0.5 g Sodium chloride, NaCl
1 g Ammonium chloride, NH4Cl
16 g Agar
1 ml Antifoam
Add dH2O to 1 liter. Autoclave.

After media is autoclaved add the following sterile ingredients:

1L Component
1 ml 1M Magnesium sulfate, MgSO4
1 ml 0.1M Calcium chloride CaCl2
10 ml 10% (w/v) glucose (or other carbon source)


This topic: Lab > ProtocolsMediaRecipes > ProtocolsRecipesM9
Topic revision: r1 - 2012-06-04 - CraigBarnhart