(r4) Barrick Lab :: ProtocolsRecipesLysogenyBroth

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---+++ LB: Luria-Bertani

_Rich media used for routine culture of E. coli and other bacteria at high cell densities._

|  *1L*  |  *5L*  |  *Component*  |
| 10 g | 50 g | Tryptone | 
| 5 g | 25 g | Yeast Extract |
| 10 g | 50 g | NaCl | 

Add dH<sub>2</sub>O to final volume. Autoclave. If mixing up large batches and aliquoting, be sure to add exact volumes to final media bottles so that they will be ready for addition of antibiotic to known concentrations.

*Note: This recipe is _different_ from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!*

Expected results: _E. coli_ strains grow to 5&times;10<sup>9</sup> cells/ml final density in LB.

Variant: 0.1&times;LB is made with 1 g Tryptone, 0.5 g Yeast Extract, and 10 g NaCl per liter. (It is 0.1&times; in the nutrients, but *not* the salt!)

Barrick Lab > ProtocolsRecipesLysogenyBroth

Contributors to this topic

JeffreyBarrick, SophiaLin, CraigBarnhart

Topic revision: r4 - 2011-07-29 - 14:07:30 - Main.CraigBarnhart

Lab.ProtocolsRecipesLuriaBertani moved from Lab.ProtocolsRecipesSalineLuriaBertani on 2011-03-08 - 14:19 by Main.JeffreyBarrick