LB: Luria-Bertani
Rich media used for routine culture of E. coli and other bacteria at high cell densities.
| 1L |
5L |
Component |
| 10 g |
50 g |
Tryptone |
| 5 g |
25 g |
Yeast Extract |
| 10 g |
50 g |
NaCl |
Add dH
2O to final volume. Autoclave. If mixing up large batches and aliquoting, be sure to add exact volumes to final media bottles so that they will be ready for addition of antibiotic to known concentrations.
Note: This recipe is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!
Expected results:
E. coli strains grow to 5×10
9 cells/ml final density in LB.
Variant: 0.1×LB is made with 1 g Tryptone, 0.5 g Yeast Extract, and 10 g NaCl per liter. (It is 0.1× in the nutrients, but
not the salt!)