LB: Luria-Bertani

1L 5L component
10 g 50 g Tryptone
5 g 25 g Yeast Extract
10 g 50 g NaCl

Add dH2O to final volume. Autoclave. If mixing up large batches and aliquoting, be sure to add exact volumes to final media bottles so that they will be ready for addition of antibiotic to known concentrations.

Note: This recipe is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

This topic: Lab > ProtocolsRecipesLysogenyBroth
Topic revision: r1 - 2011-03-08 - JeffreyBarrick
 
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