<noautolink> ---+++ ABMS: Acinetobacter Minimal Succinate Courtesy of the Averhoff lab, with modifications for available reagents. To make standard ABMS, combine the following sterilized ingredients at room temperature: | *1L* | *5L* | *Component* | | 880mL | 4.4L | diH<sub>2</sub>O | | 20mL | 100mL | 1M Sodium Succinate | | 50mL | 250mL | 20X Mineral Solution | | 50mL | 250mL | 20X Phosphate Buffer | To make ABMS plates: Add 16g/L agar and 1mL/L antifoam to diH<sub>2</sub>O, then autoclave in a 2L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55C in a water bath before combining with agar to reduce the temperature difference when combined. ---++++ ABMS Component Recipes *SL9 (trace minerals)* | *1L* | *Component* | | 800mL | diH<sub>2</sub>O | | 12.8g | Nitrotriacetic acid | | 2g | FeSO<sub>4</sub>•7H<sub>2</sub>O (note: first dissolve in 2M HCl) | | 104mg | CoCl<sub>2</sub> (anhydrous) | | 122mg | MnCl<sub>2</sub>•4H<sub>2</sub>O | | 70mg | ZnCl<sub>2</sub> | | 36mg | NaMoO<sub>4</sub>•2H<sub>2</sub>O | | 13mg | NiCl<sub>2</sub> | Add 1mL of 10mL 10X solution: | 60mg | H<sub>3</sub>BO<sub>3</sub> | | 20mg | CuCl<sub>2</sub>•2H<sub>2</sub>O | Bring to 1000mL and pH 6.5. Note: this requires a lot of NaOH, so leave room when bringing up to volume. *Mineral Solution (20X)* | *500mL* | *Component* | | 10g | NH<sub>4</sub>Cl | | 5.9g | MgSO<sub>4</sub>•7H<sub>2</sub>O | | 1g | KNO<sub>3</sub> | | 20mg | (NH<sub>4</sub>)<sub>6</sub>Mo<sub>7</sub>O<sub>24</sub>•4H<sub>2</sub>O | | 931.5µL | CaCl<sub>2</sub> Solution | | 10mL | SL9 | Bring to 500mL in a graduated cylinder with deionized water. *Phosphate Buffer (20X)* | *500mL* | *Components* | | 68g | KH<sub>2</sub>PO<sub>4</sub> | | 132.5g | Na<sub>2</sub>HPO<sub>4</sub>•H<sub>2</sub>O | | 400mL | Deionized water, pH 6.8 | Bring to 500mL.
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Topic revision: r2 - 2016-07-20 - KyleDugan