ProtocolsPythonSnippets < Lab

---+ Python snippets for biology
Requires BioPython to be installed

---++ Open common formats:
.gbk / .gbf / .gb
%CODE{"python"}%
import SeqIO
with open('/my/path/file.gb','r') as file_handle:
    record_dict = SeqIO.to_dict(SeqIO.parse(file_handle, 'gb'))
gbkFile = record_dict[list(record_dict.keys())[0]]
# the above is if there is only 1 record in the GBK file
# if multiple files are in the record, such as a genome&plas,
# this will only extract the first record

%ENDCODE%

.fa / .fasta / .fna
%CODE{"python"}%
import SeqIO
fastaList = list(SeqIO.parse("path/file.fasta", "fasta"))
%ENDCODE%

---++ Write common formats:

.gbk / .gbf / .gb
%CODE{"python"}%
import SeqIO
SeqIO.write(seqRecordObj_or_list,'/my/path/file.gbk', 'gb')
%ENDCODE%

.fa / .fasta / .fna
%CODE{"python"}%
import SeqIO
SeqIO.write(seqRecordObj_or_list,'/my/path/file.fasta', 'fasta')
%ENDCODE%

---++ local BLASTing from Python

Also requires NCBI BLAST command line software, a local BLAST database, and Pandas
%CODE{"python"}%
from Bio.Seq import Seq
from Bio import SeqIO
from Bio.SeqRecord import SeqRecord
import subprocess
from tempfile import NamedTemporaryFile
import pandas as pd

def BLAST(seq, db = 'nr_db', type = "p"):
    # 'seq' is a sequence (as a str) of a protein or nucleotide sequence
    # 'db' points to location of local BLAST database
    # 'type' specifies the type of BLAST (e.g. 'n', 'p', 'x', etc)

    query = NamedTemporaryFile()
    tmp = NamedTemporaryFile()
    SeqIO.write(SeqRecord(Seq(seq), id="temp"), query.name, "fasta")

    flags = 'qstart qend sseqid sframe pident slen sseq length sstart send qlen'
    # 'flags' specifies the specific outputs for 'output format 6' in the BLAST CL software
    extras = "-max_target_seqs 20000 -culling_limit 10 -perc_identity 75"
    # 'extras' are further flags that can be called on the CL

    subprocess.call( #the actual CL BLAST
            (f'blast{type} -query {query.name} -out {tmp.name} '
            f'-db {db} {extras} -word_size {str(wordsize)} -outfmt "6 {flags}"'),
            shell=True)

    with open(tmp.name, "r") as file_handle:  #opens BLAST file
        align = file_handle.readlines()

    tmp.close()
    query.close()

    df = pd.DataFrame([ele.split() for ele in align], columns = flags.split())
    df = df.apply(pd.to_numeric, errors='ignore')
    # puts the output of BLAST into a tidy Pandas dataframe

    return df
%ENDCODE%



-- %USERSIG{MattMcGuffie - 2020-12-03}%

---++ Comments

%COMMENT%
This topic: Lab > ComputationList > ProtocolsPythonSnippets
Topic revision: r1 - 2020-12-03 - MattMcGuffie