Barrick Lab :: ProtocolsMobilityMedia

---+++ MOB: Mobility Media

This media is useful for enhancing the mobility of ADP1, which moves across the plate by "twitching".

%TABLE{}%
|  *1L*  |  *Final [ ]*  |  *Component*  |
|  5 g   | 0.5% | agarose | 
|  5 g   | 2.0% | tryptone | 
| 2.5 g | 10 mM | NaCl |

After pouring your gel, let it set overnight at room temperature. The next day, spot with 1µl of your overnight-grown culture, leave the plate to dry for 15 minutes at room temperature, wrap in Parafilm, invert, and incubate at 30°C. You should see spreading over the next 24 hours.