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 <<O>>  Difference Topic WebRss (4 - 2005-03-28 - TWikiContributor)
 
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<		 TWiki's Lab web
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>		TWiki's Lab web
  https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsLabelingAndTech https://barricklab.org/twiki/bin/view/Lab/ProtocolsLabelingAndTech Labeling Glassware and Other Containers All bottles, flasks, tubes, graduated cylinders, and beakers that contain liquid or any substance must be labeled with a description... (last changed by AlexaMorton) 2024-07-18T16:25:04Z AlexaMorton ProcedureDNASequencing https://barricklab.org/twiki/bin/view/Lab/ProcedureDNASequencing Sanger DNA Sequencing Go to the UT DNA Sequencing Facility website. This page explains the pricing for various orders and the methods by which samples should be prepared... (last changed by ZuberiAshraf) 2023-02-27T18:32:51Z ZuberiAshraf ProtocolsOrderingPrimers https://barricklab.org/twiki/bin/view/Lab/ProtocolsOrderingPrimers Ordering Primers 1 Go to ICMB`s IDT webpage ICMB`s IDT page 1 Click Set up new Account tab on bottom left. 1 Fill out all of your information, including... (last changed by JeffreyBarrick) 2022-09-26T21:55:23Z JeffreyBarrick PhenolChloroformExtraction https://barricklab.org/twiki/bin/view/Lab/PhenolChloroformExtraction Phenol/chloroform extraction Extracting genomic bacterial DNA from pellet with phenol/chloroform with a combined EtOH precipitation step for salt/SDS/small nucleic... (last changed by MattMcGuffie) 2022-07-20T16:42:59Z MattMcGuffie RefGeneqPCR https://barricklab.org/twiki/bin/view/Lab/RefGeneqPCR C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Template Material Use cDNA dilution determined in cDNA Concentration qPCR Note: Once you... (last changed by KateElston) 2022-07-19T22:56:58Z KateElston LinkList https://barricklab.org/twiki/bin/view/Lab/LinkList Barrick Lab :: Links Advice for graduate students (Saw this linked by Cooper) for Young Scientists (Andrew Hendry) Serial Mentor (Claus Wilke`s Blog... (last changed by JeffreyBarrick) 2018-12-10T14:21:54Z JeffreyBarrick DenaturingFormaldehydeGels https://barricklab.org/twiki/bin/view/Lab/DenaturingFormaldehydeGels Denaturing Formaldehyde Gels for Verifying RNA size RNA can be sized correctly on an agarose gel. However, it needs to be denatured and then remain denatured during... (last changed by SimonDAlton) 2018-11-09T16:02:38Z SimonDAlton ProtocolsRecipesM9 https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesM9 M9 Minimal Media Plates As with DM and MG media, make sure to autoclave the agar and phosphate separately. For 1 liter of media: 1L Component 6 g... (last changed by DaciaLeon) 2018-03-27T21:25:09Z DaciaLeon

 <<O>>  Difference Topic WebRss (3 - 2005-03-28 - TWikiContributor)
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<		 TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsLabelingAndTech https://barricklab.org/twiki/bin/view/Lab/ProtocolsLabelingAndTech Labeling Glassware and Other Containers All bottles, flasks, tubes, graduated cylinders, and beakers that contain liquid or any substance must be labeled with a description... (last changed by AlexaMorton) 2024-07-18T16:25:04Z AlexaMorton ProcedureDNASequencing https://barricklab.org/twiki/bin/view/Lab/ProcedureDNASequencing Sanger DNA Sequencing Go to the UT DNA Sequencing Facility website. This page explains the pricing for various orders and the methods by which samples should be prepared... (last changed by ZuberiAshraf) 2023-02-27T18:32:51Z ZuberiAshraf PhenolChloroformExtraction https://barricklab.org/twiki/bin/view/Lab/PhenolChloroformExtraction Phenol/chloroform extraction Extracting genomic bacterial DNA from pellet with phenol/chloroform with a combined EtOH precipitation step for salt/SDS/small nucleic... (last changed by MattMcGuffie) 2022-07-20T16:42:59Z MattMcGuffie ExperimentalqPCR https://barricklab.org/twiki/bin/view/Lab/ExperimentalqPCR C1 Condition 1, C2 Condition 2, and BR# Biological Replicate Conditions If you want to be extra safe you should also include a negative control (usually... (last changed by KateElston) 2022-07-19T23:00:43Z KateElston DenaturingFormaldehydeGels https://barricklab.org/twiki/bin/view/Lab/DenaturingFormaldehydeGels Denaturing Formaldehyde Gels for Verifying RNA size RNA can be sized correctly on an agarose gel. However, it needs to be denatured and then remain denatured during... (last changed by SimonDAlton) 2018-11-09T16:02:38Z SimonDAlton ProtocolsRecipesM9 https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesM9 M9 Minimal Media Plates As with DM and MG media, make sure to autoclave the agar and phosphate separately. For 1 liter of media: 1L Component 6 g... (last changed by DaciaLeon) 2018-03-27T21:25:09Z DaciaLeon JournalClubArchives https://barricklab.org/twiki/bin/view/Lab/JournalClubArchives Barrick Lab Journal Club Archive April 13, 2015 Dacia : Reijns, Martin A M, Harriet Kemp, James Ding, Sophie Marion de Proc... (last changed by ColinBrown) 2015-04-15T14:18:16Z ColinBrown ProtocolsRecipesSpecialMedia https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSpecialMedia Special Media (last changed by CraigBarnhart) 2013-08-21T18:45:57Z CraigBarnhart
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>		 TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Enterprise. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsRecipesDavisMingioli https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesDavisMingioli DM: Davis Mingioli Growth medium used by the long term E. coli evolution experiment. 0.5L 1L 4.5L 5L Component MW 2.67 g... (last changed by AlexaMorton) 2025-05-20T18:22:19Z AlexaMorton LabNotebooks https://barricklab.org/twiki/bin/view/Lab/LabNotebooks Lab Notebook Best Practices Notebook Setup Preferred Platform: Benchling Electronic Notebooks Have an administrator add you to the Barrick Lab organization on Benchling... (last changed by JeffreyBarrick) 2024-05-08T12:38:27Z JeffreyBarrick ProtocolsAcinetobacterBaylyiADP1 https://barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterBaylyiADP1 Acinetobacter baylyi ADP1 Overview ADP1 Resources Genome Sequences type strain (GenBank Format) In general, use this genome as a reference when referring... (last changed by IsaacGifford) 2022-03-22T18:35:13Z IsaacGifford ProtocolGrofit https://barricklab.org/twiki/bin/view/Lab/ProtocolGrofit Calculating Growth Rates with Grofit The following are instructions for calculating growth rates using Grofit, an R package that is no longer supported by the current... (last changed by IsaacGifford) 2021-11-03T21:46:59Z IsaacGifford RNASeq https://barricklab.org/twiki/bin/view/Lab/RNASeq RNAseq Library Preparation Use RNA from RNASnap and Zymo column purification as outlined on wiki. rRNA Depletion Use the Ribozero (RZ) rRNA Removal Kit (Gram negative... (last changed by GabrielSuarez) 2021-05-10T17:51:17Z GabrielSuarez ProtocolsRecipesBloodHeartInfusion https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesBloodHeartInfusion Blood Heart Infusion Agar Heart Infusion Agar supplemented with sterile Sheep`s Blood. Used for the cultivation of fastidious organisms (Bee gut microbiome species... (last changed by SeanLeonard) 2017-05-15T18:34:56Z SeanLeonard RAnimation https://barricklab.org/twiki/bin/view/Lab/RAnimation Animations Saves a series of generated images to GIF, Flash, video, HTML, or PDF (LaTeX) formats. Installation install.packages(`animation`) library(animation)... (last changed by StellaWang) 2017-02-23T21:53:58Z StellaWang FormerFrontPageImages https://barricklab.org/twiki/bin/view/Lab/FormerFrontPageImages Former Front Page Images Genome dynamics in experimental evolution Read article at NRG Flying Spaghetti Monster meet syntheticbiology? Caffeine addicted E. coliSupport... (last changed by JeffreyBarrick) 2016-01-24T05:22:08Z JeffreyBarrick
 

 <<O>>  Difference Topic WebRss (2 - 2004-08-16 - PeterThoeny)
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<		 TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsMediaRecipes https://barricklab.org/twiki/bin/view/Lab/ProtocolsMediaRecipes Media Recipes When making media, make sure the autoclave bin you`re using contains water (DI or tap) at least half an inch deep to help prevent excess water loss... (last changed by AlexaMorton) 2025-06-12T14:23:46Z AlexaMorton ProtocolsRecipesMinimalGlucose https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesMinimalGlucose MG: Minimal Glucose agar, aka DM: Davis Mingioli agar Note: When making these plates it is necessary to prepare and autoclave the 3 main parts (salts, agar, and sugar... (last changed by AlexaMorton) 2025-01-09T20:14:37Z AlexaMorton ToolsBacterialGenomeResequencing https://barricklab.org/twiki/bin/view/Lab/ToolsBacterialGenomeResequencing breseq breseq is a computational pipeline for finding mutations relative to a reference sequence in short read DNA re sequencing data for haploid microbial sized... (last changed by JeffreyBarrick) 2024-10-14T00:11:08Z JeffreyBarrick ProtocolsComputerSetup https://barricklab.org/twiki/bin/view/Lab/ProtocolsComputerSetup Computer Setup for Bioinformatics and Computational Biology So, you want to harness the immense power of bioinformatics and computational biology for your science... (last changed by JeffreyBarrick) 2024-07-09T21:10:27Z JeffreyBarrick CommandLineBox https://barricklab.org/twiki/bin/view/Lab/CommandLineBox Copying files to/from UT Box at the command line If you use SSO (through UT Box), you need to first up an external password. You will need LFTP installed on your system... (last changed by JeffreyBarrick) 2024-05-18T01:40:29Z JeffreyBarrick MicroplateReaderQuickStartGuide https://barricklab.org/twiki/bin/view/Lab/MicroplateReaderQuickStartGuide Microplate Reader Quick Start Best Practices 1 Follow typical best practice for experimental design. Utilize technical replicates from the same biological sample... (last changed by JeffreyBarrick) 2024-04-17T18:27:05Z JeffreyBarrick ProtocolsReagentsPfuSso7d https://barricklab.org/twiki/bin/view/Lab/ProtocolsReagentsPfuSso7d Dear internet, We don`t distribute this plasmid. If you want to purify your own enzymes to save money, here are some options to investigate: Bioeconomy Lab ... (last changed by JeffreyBarrick) 2023-11-12T23:19:57Z JeffreyBarrick FreezerFailure https://barricklab.org/twiki/bin/view/Lab/FreezerFailure Sensaphone Protocol The sensaphone is the alarm monitor connected to both of the 80C freezers in MBB. Below is an overview of the current settings and the expected... (last changed by DanielDeatherage) 2023-01-11T20:06:04Z DanielDeatherage
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>		 TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsRecipesSOB https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSOB SOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by AlexaMorton) 2025-07-03T16:59:00Z AlexaMorton ReferenceList https://barricklab.org/twiki/bin/view/Lab/ReferenceList Barrick Lab :: Reference Information Getting Started in Lab Getting Started with Lab Techniques Synthetic Biology 101 Background on what synthetic biology... (last changed by IraZibbu) 2025-03-20T20:47:39Z IraZibbu ProtocolsConjugation https://barricklab.org/twiki/bin/view/Lab/ProtocolsConjugation General Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol... (last changed by KadenaCope) 2024-09-25T18:17:22Z KadenaCope ProtocolsLabeling https://barricklab.org/twiki/bin/view/Lab/ProtocolsLabeling Labeling Glassware and Other Containers All bottles, flasks, tubes, graduated cylinders, and beakers that contain liquid or any substance must be labeled with a description... (last changed by SophiaLin) 2024-07-09T21:53:26Z SophiaLin ProtocolsUVLibrary https://barricklab.org/twiki/bin/view/Lab/ProtocolsUVLibrary UV mutagenesis of Bacteria Determination of Optimal UV treatment This procedure is used to determine optimal treatment which will be used for library generation.... (last changed by TylerDeJong) 2024-06-05T03:03:39Z TylerDeJong ProceduresGeneGorgingEvolvedAlleles https://barricklab.org/twiki/bin/view/Lab/ProceduresGeneGorgingEvolvedAlleles Gene Gorging Evolved Alleles This procedure can be used to directly create unmarked mutations in the E. coli genome. Transform Gene Gorging and Donor Plasmid... (last changed by JeffreyBarrick) 2024-01-12T17:09:54Z JeffreyBarrick ProtocolsBTKAssembleSingleTUPlasmid https://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid Back to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by KateElston) 2021-11-03T20:57:10Z KateElston ProtocolsAcinetobacterPuddleTransformation https://barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterPuddleTransformation `In plate` / solid medium transformation of Acinetobacter baylyi ADP1 The following protocol is for single colony `in situ` (in plate) transformation with minimal... (last changed by IsaacGifford) 2021-05-13T15:52:23Z IsaacGifford
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 <<O>>  Difference Topic WebRss (1 - 2003-01-30 - PeterThoeny)
  TWiki's Lab web https://barricklab.org/twiki/bin/view/Lab The Lab web of TWiki. TWiki is a Web-Based Collaboration Platform for the Corporate World. en-us Copyright 2025 by contributing authors TWiki Administrator [admin@barricklab.org] The contributing authors of TWiki TWiki Home - this site is powered by TWiki(R).Lab https://barricklab.org/twiki/bin/view/Lab /twiki/pub/TWiki/TWikiLogos/T-logo-140x40-t.gif WebTopMenu https://barricklab.org/twiki/bin/view/Lab/WebTopMenu Top Menu of Lab Web This topic defines the menu structure of the Lab web, used by the TopMenuSkin . Barrick Lab Homepage Contact Information... (last changed by JeffreyBarrick) 2025-09-05T18:23:35Z JeffreyBarrick WebHome https://barricklab.org/twiki/bin/view/Lab/WebHome .within page container{ display: flex; flex wrap: wrap; / allows stacking when narrow / gap: 1rem; / space between blocks / background... (last changed by JeffreyBarrick) 2025-09-01T17:23:56Z JeffreyBarrick FrontPageImages https://barricklab.org/twiki/bin/view/Lab/FrontPageImages Attach images that can be used throughout the Wiki here. Barrick Lab Overview is linked to on the UT CNS website, so don`t move it! (last changed by JeffreyBarrick) 2025-09-01T11:41:29Z JeffreyBarrick PublicationList https://barricklab.org/twiki/bin/view/Lab/PublicationList Barrick Lab :: Publications Barrick Lab Publications on Google Scholar Barrick Lab researchers Equal contributions ^Corresponding author(s) Undergraduate... (last changed by JeffreyBarrick) 2025-08-31T14:27:55Z JeffreyBarrick TopMenuSkinTopBar https://barricklab.org/twiki/bin/view/Lab/TopMenuSkinTopBar Top Bar of TopMenuSkin Top bar of TopMenuSkin, replacing WebTopBar. var twTopMenuBarCloseTimer null; var twTopMenuBarTimerMsec 1000; function twToggleTopMenuBar... (last changed by JeffreyBarrick) 2025-08-29T11:39:17Z JeffreyBarrick LabPersonnel https://barricklab.org/twiki/bin/view/Lab/LabPersonnel Barrick Lab :: Team Past members of the Barrick lab Interested in our research? Join Us Principal Investigator Prof. Jeffrey E. Barrick... (last changed by JeffreyBarrick) 2025-08-29T11:15:46Z JeffreyBarrick WebTopBar https://barricklab.org/twiki/bin/view/Lab/WebTopBar (last changed by JeffreyBarrick) 2025-08-29T01:24:36Z JeffreyBarrick ContactInformation https://barricklab.org/twiki/bin/view/Lab/ContactInformation Barrick Lab :: Contact Information Jeffrey E. Barrick, PhD Hannah Professor Department of Microbiology, Genetics, Immunology Department of Entomology Michigan... (last changed by JeffreyBarrick) 2025-08-15T16:29:20Z JeffreyBarrick ProtocolsRecipesSOB https://barricklab.org/twiki/bin/view/Lab/ProtocolsRecipesSOB SOB/SOC: Super Optimal Broth For SOB: 200mL 250mL 1L Final Component 1 g 1.25 g 5 g 0.5% yeast extract... (last changed by AlexaMorton) 2025-07-03T16:59:00Z AlexaMorton ReferenceList https://barricklab.org/twiki/bin/view/Lab/ReferenceList Barrick Lab :: Reference Information Getting Started in Lab Getting Started with Lab Techniques Synthetic Biology 101 Background on what synthetic biology... (last changed by IraZibbu) 2025-03-20T20:47:39Z IraZibbu ProtocolsConjugation https://barricklab.org/twiki/bin/view/Lab/ProtocolsConjugation General Conjugation Protocol Return to BTK Page Conjugation is a reliable, robust method to transfer plasmids between bacteria. This is a general purpose protocol... (last changed by KadenaCope) 2024-09-25T18:17:22Z KadenaCope ProtocolsLabeling https://barricklab.org/twiki/bin/view/Lab/ProtocolsLabeling Labeling Glassware and Other Containers All bottles, flasks, tubes, graduated cylinders, and beakers that contain liquid or any substance must be labeled with a description... (last changed by SophiaLin) 2024-07-09T21:53:26Z SophiaLin ProtocolsUVLibrary https://barricklab.org/twiki/bin/view/Lab/ProtocolsUVLibrary UV mutagenesis of Bacteria Determination of Optimal UV treatment This procedure is used to determine optimal treatment which will be used for library generation.... (last changed by TylerDeJong) 2024-06-05T03:03:39Z TylerDeJong ProceduresGeneGorgingEvolvedAlleles https://barricklab.org/twiki/bin/view/Lab/ProceduresGeneGorgingEvolvedAlleles Gene Gorging Evolved Alleles This procedure can be used to directly create unmarked mutations in the E. coli genome. Transform Gene Gorging and Donor Plasmid... (last changed by JeffreyBarrick) 2024-01-12T17:09:54Z JeffreyBarrick ProtocolsBTKAssembleSingleTUPlasmid https://barricklab.org/twiki/bin/view/Lab/ProtocolsBTKAssembleSingleTUPlasmid Back to Golden Gate Protocols First Stage Plasmid (Transcriptional Unit) Assembly Once you have all your desired part plasmids built you can assemble them into Transcriptional... (last changed by KateElston) 2021-11-03T20:57:10Z KateElston ProtocolsAcinetobacterPuddleTransformation https://barricklab.org/twiki/bin/view/Lab/ProtocolsAcinetobacterPuddleTransformation `In plate` / solid medium transformation of Acinetobacter baylyi ADP1 The following protocol is for single colony `in situ` (in plate) transformation with minimal... (last changed by IsaacGifford) 2021-05-13T15:52:23Z IsaacGifford

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