Revision 242018-10-24 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	host	ref	description
pCA24N	Cam^R			Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector

Changed:

<
<

pACYC184

Tet^R, Cam^R

ER2420

Chang&Cohen1978

Lenski evolution experiment

>
>

pACYC184

Tet^R, Cam^R

ER2420

Chang&Cohen1978

pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map
pCP20	Amp^R	TS	BT340	FLP recombinase protocol Baba 2006	Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection.
pMMB67EH	Amp^R	RSF1010		ATCC 37622	Broad host range conjugative plasmid

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Origins of replication and copy numbers of various plasmids and cosmids - Qiagen

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
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META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 232016-02-18 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	host	ref	description
pCA24N	Cam^R			Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		ER2420	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map
pCP20	Amp^R	TS	BT340	FLP recombinase protocol Baba 2006	Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection.

Added:

>
>

pMMB67EH

Amp^R

RSF1010

ATCC 37622

Broad host range conjugative plasmid

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Origins of replication and copy numbers of various plasmids and cosmids - Qiagen

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
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META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 222014-05-12 - DanielDeatherage

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	host	ref	description
pCA24N	Cam^R			Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		ER2420	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map

Changed:

<
<

pCP20

BT340

>
>

pCP20

Amp^R

TS

BT340

FLP recombinase protocol Baba 2006

Temperature sensitive origin, FLP recombinase. Commonly used for removal of KAN cassette from Keio collection.

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Origins of replication and copy numbers of various plasmids and cosmids - Qiagen

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 212014-02-11 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	host	ref	description

Changed:

<
<

pCA24N

Cam^R

Kitagawa2005

ASKA collection vector

>
>

pCA24N

Cam^R

Kitagawa2005

ASKA collection vector

pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		ER2420	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.
pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map
pCP20			BT340

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Useful Links

Origins of replication and copy numbers of various plasmids and cosmids - Qiagen

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 202013-11-01 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

Changed:

<
<

plasmid	markers	origin	ref	description
pCA24N	Cam^R		Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS		Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R			TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R		Link1997	Church vectors for creating deletions; note: Grow at 30 degrees Celsius
pRA300, pRA301, pRA302	Spec^R		Akakura2002	Broad host range, promoterless lacZ map

>
>

plasmid	markers	origin	host	ref	description
pCA24N	Cam^R				Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	?	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD78	Cam^R	TS			Identical plasmid to pKD46, but with CAM resistance instead of AMP.
pKD3	Cam^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		BW25141	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R				TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		ER2420	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R			Link1997	Church vectors for creating deletions; note: Grow at 30° Celsius
pACBSR	Cam^R	p15A		Herring2003	Low copy number. Use a midi or maxiprep.

Added:

>
>

pRA300, pRA301, pRA302	Spec^R			Akakura2002	Broad host range, promoterless lacZ map
pCP20			BT340

Added:

>
>

Plasmids listed on this page reside in the common plasmid box in the –80°C freezers in both Welch and MBB labs.

Changed:

<
<

Common Plasmid Box

>
>

Useful Links

Origins of replication and copy numbers of various plasmids and cosmids - Qiagen

Deleted:

<
<

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid	strain
pACBSR	TOP10
pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Akakura2002	Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 192013-03-25 - DanielDeatherage

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	ref	description
pCA24N	Cam^R		Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.

Added:

>
>

pKD78

Cam^R

TS

Identical plasmid to pKD46, but with CAM resistance instead of AMP.

pKD3	Cam^R	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R		TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Link1997	Church vectors for creating deletions; note: Grow at 30 degrees Celsius
pRA300, pRA301, pRA302	Spec^R	Akakura2002	Broad host range, promoterless lacZ map

Common Plasmid Box

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid	strain
pACBSR	TOP10
pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Akakura2002	Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 182012-09-19 - CraigBarnhart

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	ref	description
pCA24N	Cam^R		Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD3	Cam^R		Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R			TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		Chang&Cohen1978	Lenski evolution experiment

Changed:

<
<

pKO3, pKOV

Suc^S, Cam^R

Link1997

Church vectors for creating deletions

>
>

pKO3, pKOV

Suc^S, Cam^R

Link1997

Church vectors for creating deletions; note: Grow at 30 degrees Celsius

pRA300, pRA301, pRA302

Spec^R

Akakura2002

Broad host range, promoterless lacZ map

Common Plasmid Box

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid	strain
pACBSR	TOP10
pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Akakura2002	Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 172012-07-17 - AlvaroRodriguez

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	ref	description
pCA24N	Cam^R		Kitagawa2005	ASKA collection vector
pKD46	Amp^R	TS	Datsenko&Wanner2000	Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.
pKD3	Cam^R		Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R		Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R			TOPO cloning kit vector
pACYC184	Tet^R, Cam^R		Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R		Link1997	Church vectors for creating deletions
pRA300, pRA301, pRA302	Spec^R		Akakura2002	Broad host range, promoterless lacZ map

Common Plasmid Box

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid	strain
pACBSR	TOP10
pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Akakura2002	Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 162012-04-08 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	ref	description
pCA24N	Cam^R		Kitagawa2005	ASKA collection vector

Changed:

<
<

pKD46

Amp^R

Datsenko&Wanner2000

Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.

>
>

pKD46

Amp^R

TS

Datsenko&Wanner2000

Wanner lambda red plasmid, temperature sensitive origin – grow at 30°C! Low copy number.

pKD3	Cam^R	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R		TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Link1997	Church vectors for creating deletions
pRA300, pRA301, pRA302	Spec^R	Akakura2002	Broad host range, promoterless lacZ map

Changed:

<
<

Plasmids currently in common plasmid box (located in -80 in both Welch and MBB)

>
>

Common Plasmid Box

Added:

>
>

Copy of box resides in –80°C freezer in both Welch and MBB.

plasmid	strain
pACBSR	TOP10
pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Akakura2002	Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"

Changed:

<
<

META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"

>
>

META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"

META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 152012-04-04 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	ref	description
pCA24N	Cam^R	Kitagawa2005	ASKA collection vector
pKD46	Amp^R	Datsenko&Wanner2000	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R	Datsenko&Wanner2000	Wanner template plasmid
pCR2.1	Kan^R, Amp^R		TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Link1997	Church vectors for creating deletions
pRA300, pRA301, pRA302	Spec^R	Akakura2002	Broad host range, promoterless lacZ map

Plasmids currently in common plasmid box (located in -80 in both Welch and MBB)

plasmid	strain

Changed:

<
<

pACBAR

TOP10

>
>

pACBSR

TOP10

pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Akakura2002	Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.
Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 142012-04-02 - CraigBarnhart

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	origin	ref	description

Changed:

<
<

pCA24N	Cam^R	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid

>
>

pCA24N	Cam^R	Kitagawa2005	ASKA collection vector
pKD46	Amp^R	Datsenko&Wanner2000	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Datsenko&Wanner2000	Wanner template plasmid
pKD13	Kan^R	Datsenko&Wanner2000	Wanner template plasmid

pCR2.1

Kan^R, Amp^R

TOPO cloning kit vector

Changed:

<
<

pACYC184	Tet^R, Cam^R		Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R		Church vectors for creating deletions
pRA300, pRA301, pRA302	Spec^R	Akakura2002	Broad host range, promoterless lacZ map

>
>

pACYC184	Tet^R, Cam^R	Chang&Cohen1978	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Link1997	Church vectors for creating deletions
pRA300, pRA301, pRA302	Spec^R	Akakura2002	Broad host range, promoterless lacZ map

Added:

>
>

Plasmids currently in common plasmid box (located in -80 in both Welch and MBB)

plasmid	strain
pACBAR	TOP10
pKD3	BW25141
pKD13	BW2514
pKD32	BW2514
pKD46	BW25141
pCP20	BT340
pRA300	?
pRA301	?
pRA302	?
pACYC184	ER2420

References

Changed:

<
<

[Akakura2002]

Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.

>
>

Akakura2002

Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.

Added:

>
>

Chang&Cohen1978	Chang, A. and Cohen, S. (1978) J. Bacteriol., 134: 1141-1156.
Datsenko&Wanner2000	Datsenko, K.A. and Wanner, B.L. (2000) Proc Natl Acad Sci U S A 97(12):6640–6645.
Kitagawa2005	Kitagawa, M. et al. (2005) DNA Res. 12: 291-9
Link1997	Link, A.J., Phillips, D, and Church, G.M. (1997) J. Bacteriol., 179(20): :6228-37.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 132012-03-28 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

Changed:

<
<

plasmid	markers	description
pCA24N	Cam^R	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

>
>

plasmid	markers	description
pCA24N	Cam^R	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

Added:

>
>

pRA300, pRA301, pRA302

Spec^R

Akakura2002

Broad host range, promoterless lacZ map

Added:

>
>

References

[Akakura2002]

Akakura, R., and Winans, S. C. (2002) J. Biol. Chem. 277:15773-15780.

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"

Added:

>
>

META FILEATTACHMENT	attachment="pRA_map.png" attr="h" comment="" date="1332900299" name="pRA_map.png" path="pRA_map.png" size="333705" stream="pRA_map.png" tmpFilename="/usr/tmp/CGItemp37190" user="JeffreyBarrick" version="1"

Revision 122012-03-27 - CraigBarnhart

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

Changed:

<
<

plasmid	markers	description
pCA24N	Cam^R	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

>
>

plasmid	markers	description
pCA24N	Cam^R	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"

Revision 112012-03-07 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	description

Changed:

<
<

pCA24N

???

ASKA collection vector

>
>

pCA24N

Cam^R

ASKA collection vector

pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

Deleted:

<
<

pkd4.gbk: pkd4.gbk

pkd4_with_gfp_sites_marked.gbk: pkd4_with_gfp_sites_marked.gbk

pKD46.gb: lambda red plasmid genbank file

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"

Changed:

<
<

META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"

>
>

META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1331154673" name="pCR2.1.gbk" path="pCR2.1.gbk" size="7254" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp39324" user="JeffreyBarrick" version="2"

META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"

Revision 102012-01-20 - BrianRenda

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	description
pCA24N	???	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

pkd4.gbk: pkd4.gbk

pkd4_with_gfp_sites_marked.gbk: pkd4_with_gfp_sites_marked.gbk

Added:

>
>

pKD46.gb: lambda red plasmid genbank file

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"

Added:

>
>

META FILEATTACHMENT	attachment="pKD46.gb" attr="" comment="lambda red plasmid genbank file" date="1327093756" name="pKD46.gb" path="pKD46.gb" size="16800" stream="pKD46.gb" tmpFilename="/usr/tmp/CGItemp30411" user="BrianRenda" version="1"

Revision 92011-12-19 - BrianRenda

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

plasmid	markers	description
pCA24N	???	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector
pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

Added:

>
>

pkd4.gbk: pkd4.gbk

pkd4_with_gfp_sites_marked.gbk: pkd4_with_gfp_sites_marked.gbk

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"

Added:

>
>

META FILEATTACHMENT	attachment="pkd4.gbk" attr="" comment="" date="1324333782" name="pkd4.gbk" path="pkd4.gbk" size="6238" stream="pkd4.gbk" tmpFilename="/usr/tmp/CGItemp30310" user="BrianRenda" version="1"
META FILEATTACHMENT	attachment="pkd4_with_gfp_sites_marked.gbk" attr="" comment="" date="1324334147" name="pkd4_with_gfp_sites_marked.gbk" path="pkd4 with gfp sites marked.gbk" size="10232" stream="pkd4 with gfp sites marked.gbk" tmpFilename="/usr/tmp/CGItemp30432" user="BrianRenda" version="1"

Revision 82011-06-13 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

Changed:

<
<

pCA24N (ASKA strain host)
pKD3 (Cam template plasmids)
pKD13 (Kan template plasmids)
pCR2.1 (TOPO cloning kit vector)
pACYC184 (Lenski evolution experiment) – tetR, cmR
pKO3, pKOV (Church Lab vectors for creating deletions) – sacB, cmR

>
>

plasmid	markers	description
pCA24N	???	ASKA collection vector
pKD46	Amp^R	Wanner lambda red plasmid, TS origin – grow at 30°C! Low copy number.
pKD3	Cam^R	Wanner template plasmid
pKD13	Kan^R	Wanner template plasmid
pCR2.1	Kan^R, Amp^R	TOPO cloning kit vector

Added:

>
>

pACYC184	Tet^R, Cam^R	Lenski evolution experiment
pKO3, pKOV	Suc^S, Cam^R	Church vectors for creating deletions

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"

Revision 72011-02-21 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

pCA24N (ASKA strain host)
pKD3 (Cam template plasmids)
pKD13 (Kan template plasmids)
pCR2.1 (TOPO cloning kit vector)
pACYC184 (Lenski evolution experiment) – tetR, cmR
pKO3, pKOV (Church Lab vectors for creating deletions) – sacB, cmR

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"

Added:

>
>

META FILEATTACHMENT	attachment="pKOV.txt" attr="" comment="" date="1298308413" name="pKOV.txt" path="pKOV.txt" size="10834" stream="pKOV.txt" tmpFilename="/usr/tmp/CGItemp17348" user="JeffreyBarrick" version="1"

Revision 62011-02-17 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

pCA24N (ASKA strain host)
pKD3 (Cam template plasmids)
pKD13 (Kan template plasmids)
pCR2.1 (TOPO cloning kit vector)
pACYC184 (Lenski evolution experiment) – tetR, cmR
pKO3, pKOV (Church Lab vectors for creating deletions) – sacB, cmR

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"

Deleted:

<
<

META FILEATTACHMENT	attachment="pKO3.txt" attr="" comment="" date="1297365196" name="pKO3.txt" path="pKO3.txt" size="8420" stream="pKO3.txt" tmpFilename="/usr/tmp/CGItemp16009" user="JeffreyBarrick" version="1"

Revision 52011-02-10 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

pCA24N (ASKA strain host)
pKD3 (Cam template plasmids)
pKD13 (Kan template plasmids)
pCR2.1 (TOPO cloning kit vector)

Changed:

<
<

pACYC184 (Lenski evolution experiment)

>
>

pACYC184 (Lenski evolution experiment) – tetR, cmR

Added:

>
>

pKO3, pKOV (Church Lab vectors for creating deletions) – sacB, cmR

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"

Added:

>
>

META FILEATTACHMENT	attachment="pKO3.txt" attr="" comment="" date="1297365196" name="pKO3.txt" path="pKO3.txt" size="8420" stream="pKO3.txt" tmpFilename="/usr/tmp/CGItemp16009" user="JeffreyBarrick" version="1"

Revision 42009-08-03 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

pCA24N (ASKA strain host)
pKD3 (Cam template plasmids)
pKD13 (Kan template plasmids)

Changed:

<
<

pCR2.1

>
>

pCR2.1 (TOPO cloning kit vector)

Added:

>
>

pACYC184 (Lenski evolution experiment)

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"

Added:

>
>

META FILEATTACHMENT	attachment="pACYC184.gbk" attr="" comment="" date="1249333784" name="pACYC184.gbk" path="pACYC184.gbk" size="7933" stream="pACYC184.gbk" tmpFilename="/usr/tmp/CGItemp52774" user="JeffreyBarrick" version="1"

Revision 32009-07-14 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Commonly Used Plasmids

Added:

>
>

pCA24N (ASKA strain host)
pKD3 (Cam template plasmids)
pKD13 (Kan template plasmids)
pCR2.1

META FILEATTACHMENT	attachment="pCA24N.gbk" attr="" comment="" date="1243603290" name="pCA24N.gbk" path="pCA24N.gbk" size="10116" stream="pCA24N.gbk" tmpFilename="/usr/tmp/CGItemp42718" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pCR2.1.gbk" attr="" comment="" date="1243603303" name="pCR2.1.gbk" path="pCR2.1.gbk" size="8323" stream="pCR2.1.gbk" tmpFilename="/usr/tmp/CGItemp42775" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD3_Cam_template.gbk" attr="" comment="" date="1243603342" name="pKD3_Cam_template.gbk" path="pKD3_Cam_template.gbk" size="6787" stream="pKD3_Cam_template.gbk" tmpFilename="/usr/tmp/CGItemp42667" user="JeffreyBarrick" version="1"
META FILEATTACHMENT	attachment="pKD13_Kan_template.gbk" attr="" comment="" date="1243603359" name="pKD13_Kan_template.gbk" path="pKD13_Kan_template.gbk" size="7753" stream="pKD13_Kan_template.gbk" tmpFilename="/usr/tmp/CGItemp42655" user="JeffreyBarrick" version="1"

Revision 22009-06-30 - JeffreyBarrick

META TOPICPARENT	name="ReferenceList"

Changed:

<
<