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| META TOPICPARENT |
name="ProtocolList" |
Isolation of Total RNA |
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- Add 2 volumes (400ul) of RNA Binding Buffer to each volume of RNA sample and mix well.
- Add 1 volume (600ul) ETOH (95-100%) to the mixture and mix well.
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- Transfer the mixture to the Zymo-Spin IIC column in a collection tube and centrifuge at >12,000 x g for 1 min. Discard the flow through.
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- Transfer half the mixture to the Zymo-Spin IIC column in a collection tube and centrifuge at >12,000 x g for 1 min. Discard the flow through.
- Load the remaining half and repeat spin, discard.
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- During this spin, prepare the DNase 1 cocktail for use. Per reaction:
- 5ul RNase-Free DNase I
- 75ul Reaction Buffer
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