Difference: ProtocolsWorkingWithVibrioNatriegens (16 vs. 17)

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Working with Vibrio natriegens (Vmax)

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At 4°C, Vibrio species reach a “viable but nonculturable” state because they are unable to detoxify the lethal reactive oxygen species that is present in the culture medium, especially in solid media. Colder temperatures reduce the activity and expression of the catalase gene. This can be prevented by supplementing the media with sodium pyruvate or catalase. Another way to circumvent this issue is by engineering Vmax with a plasmid that either (1) contains an E coli operon for the catalase gene, or (2), encodes for the E coli gene that produces catalase.
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At 4°C, Vibrio species reach a “viable but nonculturable” state because they are unable to detoxify the lethal reactive oxygen species that is present in the culture medium, especially in solid media. Many bacteria naturally produce highly reactive hydrogen peroxide as they oxidize flavoprotein with oxygen, and hydrogen peroxide damages cell parts. Some species can produce catalase to break down H2O2, and while Vmax does carry the catalase gene, colder temperatures reduce the gene's activity and expression. This can be prevented by supplementing the media with sodium pyruvate or catalase. Another way to circumvent this issue is by engineering Vmax with a plasmid that either (1) contains an E coli operon for the catalase gene, or (2), encodes for the E coli gene that produces catalase.
 
Natural DNase Production
 
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