Difference: ProtocolsSsdnaRecombination (4 vs. 5)

Revision 52013-03-27 - DanielDeatherage

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META TOPICPARENT name="ProtocolList"

λ red mediated ssDNA gene modification

Background

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  1. Mutation Sequencing Oligo:
    • Primer to be used for final sequencing to verify mutation on correct background.
    • Primer should be ~20bp long, ~150+ bp upstream of Mutation screen oligo.
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Recombineering.png
 

Make λ red electrocompetent cells

  1. Make the line you wish to have your mutation of interest electrocompetent
 
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