Difference: ProtocolsSsdnaRecombination (3 vs. 4)

Revision 42013-03-27 - DanielDeatherage

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META TOPICPARENT name="ProtocolList"

λ red mediated ssDNA gene modification

Background

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    • Primer to be used for final sequencing to verify mutation on correct background.
    • Primer should be ~20bp long, ~150+ bp upstream of Mutation screen oligo.
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Make λ red electrocompetent cells

  1. Make the line you wish to have your mutation of interest electrocompetent
    • ProtocolsElectrocompetentCells
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  1. Submit sample for sequencing with either primer.

-- Main.DanielDeatherage - 25 Mar 2013

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