Difference: ProtocolsRestrictionEnzymeCloning (4 vs. 5)

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Restriction Enzyme Cloning

Restriction enzyme cloning is a bread-and-butter technique in molecular biology for modifying plasmids to contain genes or other DNA sequences of interest. While it may be more time consuming than some recently developed techniques, it is very reliable.

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For background on restriction enzyme cloning and some pretty pictures, check out the wiki on the topic, and check out the useful diagram on this Chinese website.
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For background on restriction enzyme cloning and some pretty pictures, check out the Wikipedia page on this topic.
 
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  1. Inoculate 5 mL of liquid LB media plus the appropriate antibiotic each with a colony with insert of correct size. Grow overnight
  2. Isolate plasmid from overnight culture using a miniprep kit.
  3. Sequence the insert using the same primers you used to verify insert size. Analyze sequence to confirm correct insert.
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-- Main.MichaelHammerling - 01 Feb 2012
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