Difference: ProtocolsRecipesTetrazoliumSugar (3 vs. 4)

Revision 42012-05-04 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolsMediaRecipes"

TA: Tetrazolium Sugar (TA, TM, TL, ...)

Combine in a 2 L flask:

1.5L Component
15 g Tryptone
1.5 g Yeast Extract
7.5 g NaCl
24 g Agar
1.5 ml 5% Antifoam
Add dH2O to 1.3 liter.

Combine in a 500 ml flask:

1.5L Component
6 g Sugar (arabinose = TA, maltose = TM, lactose = TL)
Add dH2O to 0.2 liter.

Autoclave sugar and media separately. Total combined volume is 1.5 liters.

Combine and add:

1.5L Component
Changed:
<
<
1.5 ml TTC (5%) or 7.5 ml TTC (1%)
>
>
1.5 ml TTC (5%)
Added:
>
>		 Source: Lenski Lab Protocol
 
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