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| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
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<
< | RCV Medium |
>
> | RCV Medium |
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<
< | RCV is a complex medium |
>
> | RCV is a complex media consisting of 3 different sub-componets that must be prepared ahead of time if they are not already made. |
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|
>
> | Main recipe |
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| 1L |
Component |
| 10 mL |
10% (NH4)2 SO4 |
| 40 mL |
10% DL-malate, pH 6.8 |
|
|
<
< |
| 50 mL |
Super salts |
| 15 mL |
0.64 KPO4, pH 6.8 |
|
>
> |
| 50 mL |
Super salts (see below) |
| 15 mL |
0.64 KPO4, pH 6.8 (see below) |
|
| | |
|
<
< | add 3/4 H2O |
>
> | add 3/4 H2O before adding the KPO4 |
|
>
> | adjust pH to 6.8 if needed
if making plates add 16 g Agar per L
0.64M KPO4, pH 6.8
| 500mL |
Component |
| 20 g |
KH2PO4 |
| 30 g |
K2HPO4 |
Super salts
| 1L |
Component |
| 40 mL |
1.0% EDTA |
| 20 mL |
20% MgSO4 5H2O |
| 20 mL |
7.5% CaCl2 2H2O |
| 20 mL |
Trace elements (see below) |
| 48 mL |
0.5% FeSO4 7H2O |
| 20 mL |
0.1% thiamine-HCl |
Trace Elements
| 250mL |
Component |
| 0.3975 g |
MnSO4H2O |
| 0.7 g |
H3BO3 (Boric Acid) |
| 0.01 g |
Cu(NO3)2 3H2O |
| 0.06 g |
ZnSO4 7H2O |
| 0.1875 g |
NaMoO4 2H2O (Sodium molybdate VI dihydrate) |
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