| |
| META TOPICPARENT |
name="ProtocolsMediaRecipes" |
|
|
<
< | |
| | M9 Minimal Media Plates |
|
<
< |
| 1L |
Component |
| 6 g |
Sodium phosphate, Na2HPO4•H2O |
|
>
> |
| 1L |
Component |
| 6 g |
Sodium phosphate, Na2HPO4 (anhydrous) |
|
| |
| 3 g |
Potassium phosphate, KH2PO4 |
| 0.5 g |
Sodium chloride, NaCl |
|
|
<
< |
| 1 g |
Ammonium chloride, NH4Cl |
|
>
> |
| 1 g |
Ammonium chloride, NH4Cl |
|
| |
|
|
<
< | Add dH2O to 1 liter. Autoclave. |
>
> | Add dH2O to 1 liter. Autoclave. |
| |
After media is autoclaved add the following sterile ingredients: |
|
<
< |
|
>
> |
|
| |
| 1 ml |
1M Magnesium sulfate, MgSO4 |
| 1 ml |
0.1M Calcium chloride CaCl2 |
|
|
<
< |
| 10 ml |
10% (w/v) glucose (or other carbon source) |
|
>
> |
| 10 ml |
10% (w/v) glucose (or other carbon source) |
|
| | |
