Difference: ProtocolsRecipesLysogenyBrothAgar (2 vs. 3)

Revision 32011-07-29 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

LB: Luria-Broth

Changed:
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<		Combine in a 2 L flask:
>
>		Combine in a 2 L flask the following:
Added:
>
>
1.5L Component
 
15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam

Add dH2O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

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