Difference: ProtocolsRecipesLysogenyBrothAgar (1 vs. 2)

Revision 22011-07-26 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

LB: Luria-Broth

Combine in a 2 L flask:

15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
Changed:
<
<
1.5 ml 5% Antifoam or .75 ml 10% Antifoam
>
>
1.5 ml 5% Antifoam
  Add dH2O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

Revision 12011-06-14 - CraigBarnhart

 
META TOPICPARENT name="ProtocolsMediaRecipes"

LB: Luria-Broth

Combine in a 2 L flask:

15 g Tryptone
7.5 g Yeast Extract
15 g NaCl
24 g Agar
1.5 ml 5% Antifoam or .75 ml 10% Antifoam

Add dH2O to 1.5 L. Autoclave.

Note: This is different from "LB" = "Luria Broth" media, which has only 5 g NaCl per liter!

To make "soft" agar for phage studies, use only 7 g agar per liter.

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