Difference: ProtocolsRecipesDR ( vs. 1)

Revision 12011-07-29 - CraigBarnhart

META TOPICPARENT	name="ProtocolsMediaRecipes"

DR: Defined minimal media for D. radiodurans

250 ml	500 ml	Component
50 ml	100 ml	5x M9 salts
250 ul	500 ul	5mM MnCl₂
250 ul	500 ul	0.8M MgCl₂
250 ul	500 ul	0.18 M CaCl₂
2.5 ml	5 ml	Syringe filtered Vitamins Mix
500 ul	1 ml	Syringe filtered Amino Acid mix (see below)

Add dH₂O to final volume.
Note: All components are either pre-sterilized or syringe filtered so the only things that need to be autoclaved are the receiving bottle and the added water.

To prepare the amino acids:

Amount	Amino Acid
1 g	L-Cys
0.5 g	L-His
0.5 g	L-Met

Dissolve these amino acids in 40 ml of dH₂O

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