Difference: ProtocolsRecipesABMS (1 vs. 7)

ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

To make standard ABMS, combine the following pre-sterilized ingredients at room temperature:

To make ABMS agar:

Per liter of media, add 16 g/L agar and 1 mL/L antifoam to diH₂O, then autoclave in a 2 L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55°C in a water bath before combining with agar to reduce the temperature difference when combined.

ABMS Component Recipes

SL9 (trace minerals)

Add 1 mL of 10 mL 10× solution:

† Substitution: You may use 465.8 µL of a 1 M CaCl₂ per 500 ml of stock solution for this component. The final concentration of Ca²⁺ in the 20× stock is 9.12 mM.

Bring to 500mL in a graduated cylinder with deionized water.

Phosphate Buffer (20×)

Bring to 500 mL with diH₂O; pH should be ~6.9-7.0 (you may add 400 mL diH₂O first and bring pH up to 6.8 then complete volume to 500mL)

* Source: Averhoff lab

ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

To make standard ABMS, combine the following pre-sterilized ingredients at room temperature:

Per liter of media, add 16 g/L agar and 1 mL/L antifoam to diH₂O, then autoclave in a 2 L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55°C in a water bath before combining with agar to reduce the temperature difference when combined.

ABMS Component Recipes

SL9 (trace minerals)

ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

To make standard ABMS, combine the following pre-sterilized ingredients at room temperature:

To make ABMS agar:

Per liter of media, add 16 g/L agar and 1 mL/L antifoam to diH₂O, then autoclave in a 2 L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55°C in a water bath before combining with agar to reduce the temperature difference when combined.

ABMS Component Recipes

SL9 (trace minerals)

Add 1 mL of 10 mL 10X solution:

Adjust pH to 6.5 using 1 M NaOH. Bring volume to 1000mL with diH₂O. Note: this requires a lot of NaOH, so leave room when bringing up to volume.

Mineral Solution (20X)

Bring to 500mL in a graduated cylinder with deionized water.

Phosphate Buffer (20X)

ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

To make standard ABMS, combine the following pre-sterilized ingredients at room temperature:

To make ABMS agar:

Per liter of media, add 16 g/L agar and 1 mL/L antifoam to diH₂O, then autoclave in a 2 L flask. When water and agar are cool enough to touch, add component solutions. Optional: warm components at 55°C in a water bath before combining with agar to reduce the temperature difference when combined.

ABMS Component Recipes

SL9 (trace minerals)

Add 1 mL of 10 mL 10X solution:

Mineral Solution (20X)

Bring to 500mL in a graduated cylinder with deionized water.

Phosphate Buffer (20X)

ABMS: Acinetobacter Minimal Succinate

Courtesy of the Averhoff lab, with modifications for available reagents.

ABMS Component Recipes

SL9 (trace minerals)

Phosphate Buffer (20X)

ABMS

ABMS stands for.... It is used for....

If making ABMS solid media also add 15 g of agar and 1 ml of 5% antifoam per liter.