Difference: ProtocolsReagentRecipes (18 vs. 19)

Revision 192022-03-22 - KateElston

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META TOPICPARENT name="ProtocolList"

Reagent and Buffer Recipes

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  Makes ~4ml. Aliquot to 1.5 ml centrifuge tubes.
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To prepare the Invitrogen 1kb+ ladder: Create a 1:10 dilution of ladder in water, accounting for the addition of your dye of choice (see example below)

100 ul Ladder
166.6 ul 6x Loading Buffer (above)
733.4 ul Milipure H2O
 

Stock solutions

Tris-HCl, 1 M

 
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