Difference:
ProtocolsReagentRecipes
(18 vs. 19)
Revision 19
2022-03-22 -
KateElston
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META TOPICPARENT
name="ProtocolList"
Reagent and Buffer Recipes
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Makes ~4ml. Aliquot to 1.5 ml centrifuge tubes.
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To prepare the Invitrogen 1kb+ ladder:
Create a 1:10 dilution of ladder in water, accounting for the addition of your dye of choice (see example below)
100 ul
Ladder
166.6 ul
6x Loading Buffer (above)
733.4 ul
Milipure H2O
Stock solutions
Tris-HCl, 1 M
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