Difference: ProtocolsPhageTiters (4 vs. 5)

Revision 52020-02-26 - JeffreyBarrick

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META TOPICPARENT name="ProtocolPhageLysate"

Determining Phage Titer

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T7-plaques-1E4.png Phage T7 plaques after overnight growth at 37C of a 104 dilution of a freezer stock. T7 plaques continue to grow. Incubate the plates at 30C if you want to slow plaque growth.
T7-plaques-1E2.png Phage T7 plaques after overnight growth at 37C of a 102 dilution of a freezer stock. The phage has lysed all bacteria in this plate. The remaining colonies (small if they are embedded in the top agar and large if they reach the surface) are derived from mutant bacterial cells that have become resistant to the phage.
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Tourbleshooting

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Troubleshooting

 
  • Top-agar layer is grainy or clumpy.
    It likely cooled too much before you added phage+cells and poured it.
 
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