Difference: ProtocolsFindStrainsPlasmids (2 vs. 3)

Revision 32018-04-06 - SeanLeonard

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 name="ProtocolList" 


 Find Strains, Plasmids, and Genes
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+Reminder: Always revive new organisms according to an established protocol and archive a lab stock of the original freeze-dried culture after it has been resuspended, in addition to the initial culture, and cultures from picked colonies.
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+ Reminder: Always revive new organisms according to an established protocol and archive a lab stock of the original freeze-dried culture after it has been resuspended, in addition to the initial culture, and cultures from picked colonies.
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+ General culture collections
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+ General culture collections
  American Type Culture Collection (ATCC)
 Note: ATCC is usually much more expensive than other sources.
  German Collection of Microorganisms and Cell Cultures (DSMZ)
  UK National Collection of Industrial Food and Marine Bacteria (NCIMB)
  USDA Agricultural Research Service Culture Collection (NRLL)
  Culture Collections Information Worldwide (WDCM) 
  This is a database of culture collections from all over the world.
 

 Specialty strain collections 
 
 E. coli Genetic Stock Center
 

 Plasmids collections 
 
 Addgene 
 Most common source for published plasmids.
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+ Registry of Standard Biological Parts
 We have the iGEM distributions from 2012 and 2014–present.
  Standard European Vector Architecture 2.0 (SEVA)
 Similar to iGEM repository, but uses a different standard that allows backbone elements (like the origin) to vary.
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+ Registry of Standard Biological Parts
 We have the iGEM distributions from 2012 and 2014–present.
  Standard European Vector Architecture 2.0 (SEVA)
 Similar to iGEM repository, but uses a different standard that allows backbone elements (like the origin) to vary.
  Advice for choosing strains 
 
 Whenever possible, choose a strain that has a complete genome sequence available. You can check by searching on the NCBI Genome Page. After you do a search, be sure to click on the link by "Organism Overview ; Genome Assembly and Annotation report [XXXX]" to see a table of all XXXX of the genomes with sequencing data. Ideally, you want ones with a level of "complete".

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