Difference: ProtocolsCulturingSnodgrassellaAlvi (3 vs. 4)

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Working with _Snodgrassella alvi

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  Snodgrassella alvi strain wkB2 (type strain) is generally cultured at 35C and 5% CO2. It grows robustly on agar plates after 48 hours on the following media:
  • Heart Infusion Agar
  • Heart Infusion Agar supplement with Sheep's Blood
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  • Columbia Agar supplement with Sheep's Blood
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  • Columbia Agar supplement with Sheep's Blood (CBA)
 
  • R2 Agar

Liquid Media

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  • R2 Liquid Media

Conjugation

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Gene disruption

-- Main.SeanLeonard - 25 Aug 2016

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Snodgrassella alvi can be conjugated using E. coli donor strains and mobilizable rsf1010-based plasmids. Refer to the Bee Microbiome Toolkit paper for full description of working with this species.
Perform conjugation as described here: Conjugation, with the following specifics: use 10:1 ratio of S. alvi to donor E. coli pooled on CBA plates. Incubate overnight, then resuspend and plate on selective CBA plates.
 
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