Difference: ProtocolsCulturingSnodgrassellaAlvi (3 vs. 4)

Revision 42018-10-25 - SeanLeonard

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META TOPICPARENT	name="ProtocolList"

Working with _Snodgrassella alvi

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Snodgrassella alvi strain wkB2 (type strain) is generally cultured at 35°C and 5% CO₂. It grows robustly on agar plates after 48 hours on the following media:

Heart Infusion Agar
Heart Infusion Agar supplement with Sheep's Blood

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Columbia Agar supplement with Sheep's Blood

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Columbia Agar supplement with Sheep's Blood (CBA)

R2 Agar

Liquid Media

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R2 Liquid Media

Conjugation

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Gene disruption

-- Main.SeanLeonard - 25 Aug 2016

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Snodgrassella alvi can be conjugated using E. coli donor strains and mobilizable rsf1010-based plasmids. Refer to the Bee Microbiome Toolkit paper for full description of working with this species.
Perform conjugation as described here: Conjugation, with the following specifics: use 10:1 ratio of S. alvi to donor E. coli pooled on CBA plates. Incubate overnight, then resuspend and plate on selective CBA plates.

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