Difference: ProtocolsCulturingSnodgrassellaAlvi (2 vs. 3)

Revision 32018-04-27 - SeanLeonard

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Working with _Snodgrassella alvi

Snodgrassella alvi is a member of the honey bee ( Apis mellifera) gut core microbiota that can be cultivated in vitro. Its isolation and characterization were recently carried out by Waldan Kwong in the laboratory of Dr. Nancy Moran and are described in depth in here.

In vitro culture conditions

Plate Growth

Snodgrassella alvi strain wkB2 (type strain) is generally cultured at 35°C and 5% CO₂. It grows robustly on agar plates after 48 hours on the following media:

Heart Infusion Agar
Heart Infusion Agar supplement with Sheep's Blood
Columbia Agar supplement with Sheep's Blood
R2 Agar

Liquid Media

Snodgrassella alvi can be cultivated in the following liquid media (same general culture conditions as above, without shaking):

Insectagro (proprietary media)
Salt-free LB
R2 Liquid Media

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Conjugation

Gene disruption

-- Main.SeanLeonard - 25 Aug 2016

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