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Acinetobacter baylyi ADP1 Overview |
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- pBTK622
Plasmid containing the tdk-kanR cassette used for genome editing.
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Working with ADP1
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- LB is typically used when growing ADP1 in rich media and Minimal Succinate (ABMS) media is used when growing ADP1 in minimal media (recipes for both medias can be found here).
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- Media can be supplemented with antibiotics for selection of strains transformed with resistance markers as described below.
- ADP1 grows at both 30°C and 37°C. The Barrick Lab typically incubates ADP1 cultures at 30°C with shaking at 200 rpm.
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