Difference: ProtocolsAcinetobacterBaylyiADP1 (5 vs. 6)

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Acinetobacter baylyi ADP1 Overview

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    • pBTK622
      Plasmid containing the tdk-kanR cassette used for genome editing.

  • Functional Genomics
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Working with ADP1

  • Culture conditions
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    • LB is typically used when growing ADP1 in rich media and Minimal Succinate (ABMS) media is used when growing ADP1 in minimal media (recipes for both medias can be found here).
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    • LB is typically used when growing ADP1 in rich media.
    • Minimal Succinate (ABMS) media is used when growing ADP1 in minimal media.
 
      • Media can be supplemented with antibiotics for selection of strains transformed with resistance markers as described below.
    • ADP1 grows at both 30C and 37C. The Barrick Lab typically incubates ADP1 cultures at 30C with shaking at 200 rpm.
 
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