Difference: ProtocolsAcinetobacterBaylyiADP1 (2 vs. 3)

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Acinetobacter baylyi ADP1 Overview

ADP1 Resources

  • Genome Sequences
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  • Other Useful Sequences
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    • pBTK622
      Plasmid containing the tdk-kanR cassette used for genome editing.
 
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Plasmids

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Common high-copy number E. coli plasmids do not work in ADP1. A synthetic plasmid (pBAV1K) and broad-host-range plasmid (RSF1010) have been used successfully. In our hands, pBAV1K is less reliable. It is also important to note that because plasmid DNA is linearizes when taken up through the competence apparatus, that transformation of plasmids can be significantly less efficient than transformations used to incorporate DNA directly in the chromosome.
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Common high-copy number E. coli plasmids do not work in ADP1.

A synthetic plasmid (pBAV1K) and broad-host-range plasmid (RSF1010) have been used successfully. In our hands, pBAV1K is less reliable. It is also important to note that because plasmid DNA is linearizes when taken up through the competence apparatus, that transformation of plasmids can be significantly less efficient than transformations used to incorporate DNA directly in the chromosome.

 
Origin Copy Number Example
ColE1 (pUC, pBR322, etc.) type plasmids Do not replicate NA
 
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