Difference: ProtocolPhageLysate (5 vs. 6)

Revision 62021-06-08 - JeffreyBarrick

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META TOPICPARENT name="ProtocolsPhageAdsorptionRate"

Phage Lysate Preparation

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We have used this protocol with phages T4, T5, T6 and T7. The general procedure should work with most E. coli phages (although lysis times and final titers may differ). See the variants section for some ways to modify it for other hosts/phages.
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We have used this protocol with phages T4, T5, T6 and T7. The general procedure should work with most lytic E. coli phages (although lysis times and final titers may differ). See the variants section for some ways to modify it for other hosts/phages.
 

Materials

  • Overnight culture of a permissive E. coli host (see the table below)
  • Frozen stock of phage (or plate with fresh plaques)
  • LB-Miller Media (w/antibiotics or supplements appropriate for host E. coli)
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  • 50 mL flasks (recommended) or test tubes
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  • 50 mL flasks (recommended) or test tubes
 
  • 15 mL Falcon tubes
  • Shaking incubator at appropriate temperature (37 or 30)
  • Chloroform (Safety: All work with chloroform should be done in a fume hood with gloves, a lab coat, and eye protection.)
 
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