Difference: ProtocolPhageLysate (4 vs. 5)

Revision 52020-02-25 - JeffreyBarrick

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META TOPICPARENT name="ProtocolsPhageAdsorptionRate"

Phage Lysate Preparation

We have used this protocol with phages T4, T5, T6 and T7. The general procedure should work with most E. coli phages (although lysis times and final titers may differ). See the variants section for some ways to modify it for other hosts/phages.
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 *REL606 is resistant to phage T6
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Procedure

 
T7-lysate.png
T7 lysate showing small amount of chloroform that has settled to the bottom of the tube.
 
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