| |
| META TOPICPARENT |
name="ProceduresTargetedSequencing" |
topA Gene Sequencing
The topA gene coordinates are 1329420-1332017.
The topA mutation in the Ara-1 long-term line is 1329516:C→T.
| primer |
who |
coords |
sequence |
| RL0398 |
TC |
1329010-1329031 |
5'‑CGCGGTCGATGGGTTGTGTCTC |
| RL0399 |
TC |
1332121-1332101 |
5'‑ATCATTTATAGCCTTAGTGTA |
| RL0400 |
TC |
1329557-1329577 |
5'‑GAGTGCCGACTCTACCTCCAC |
| RL0401 |
TC |
1330088-1330109 |
5'‑TGCCAGCACGACCACGCCATCT |
| RL0402 |
TC |
1330609-1330629 |
5'‑AGTTCGTTGCCTGCCAGATGA |
| RL0403 |
TC |
1331103-1331122 |
5'‑GCTGTACTGGATCACTTCTT |
| RL0410, RL0610 |
RW, BW |
1329489-1329508 |
5'‑GACTACGTGGTGAAATCCAG |
| RL0411, RL0611 |
RW, BW |
1329683-1329663 |
5'‑ACCAGGCAACACTTCATAGTG |
| RL0738 |
CO |
1329010-1329029 |
5'‑CGCGGTCGATGGGTTGTGTC |
| RL0739 |
CO |
1332323-1332302 |
5'‑TCCCGGGTTGTGATGTGTAAAG |
| RL0740 |
CO |
1329648-1329670 |
5'‑CACAATTGGGAGGCGCACTATGA |
| RL0741 |
CO |
1331694-1331672 |
5'‑GTAATGGCACCGGATCTTCTTTC |
| RL0742 |
CO |
1329990-1330007 |
5'‑GCCTGTCTGCCGGTCGTGTG |
| RL0743 |
CO |
1331055-1331032* |
5'‑TCTGCGCGGTAAAgTCGTAGTTCA |
* This primer has a base mismatch (lowercase) to REL606. It must have been designed based on the MG1655 sequence.
All coordinates are for the reference strain REL606. |
