Difference: ProceduresPolyacrylamideGelElectrophoresis (7 vs. 8)

Revision 82013-06-07 - AlvaroRodriguez

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META TOPICPARENT name="ProtocolList"

Polyacrylamide Gel Electrophoresis

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  Note: Many recipes for this do not add the TBE buffer or substitute sucrose for glycerol.
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6x Urea Loading Buffer

24 g Urea
2 ml 0.5 M EDTA
100 Ál 1 M Tris pH 7.5
16 mg Bromophenol Blue

Add ddH2O to 50 ml. Use for Use for denaturing gels.

Note: Bromophenol Blue is optional.

 

10x TBE Buffer

108 g Tris base
 
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