Difference: ProceduresKappaNgsPrep (32 vs. 33)

Revision 332018-09-27 - GabrielSuarez

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Major version notes

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This is a protocol based on Kappa LTP Preparation Kit manual KR0453 - v3.13
The main difference centers around the use of 1/2 volume in each reaction so as to get twice as many sample preps for the same price.
This was validated on 2 extremely similar samples from Gabo by DED in July of 2014, and the final data was indistinguishable between the 1x volume sample and the 0.5x volume sample.
This has subsequently been used in more than a dozen (4-15-15) other preps without incident.
The default fragmentation protocol is now NEB fragmentase rather than Covaris.
This protocol is for standard applications. Please click here for rare variant within a population protocol.
>
>
This is a protocol based on Kapa LTP Preparation Kit manual KR0453 - v3.13
The main difference centers around the use of 1/2 volume in each reaction so as to get twice as many sample preps for the same price.
This was validated on 2 extremely similar samples from Gabo by DED in July of 2014, and the final data was indistinguishable between the 1x volume sample and the 0.5x volume sample.
This has subsequently been used in more than a dozen (4-15-15) other preps without incident.
The default fragmentation protocol is now NEB fragmentase rather than Covaris.
This protocol is for standard applications. Please click here for rare variant within a population protocol.
 

Before Beginning and Important Notes

  1. Low retention (maximum recovery) tubes and filter tips should be used at every step. Regular tips commonly used for EtOH removal during wash steps.
Line: 260 to 260
 

ERROR rates

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incorporate remaining information from full page into optional information here. old page
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incorporate remaining information from full page into optional information here. old page
  -- Main.DanielDeatherage - 09 Jan 2015
-- Gabriel Suarez - 28 Ago 2018
 
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