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Summary of the Known Idiosyncrasies of Each Long-Term Line | ||||||||
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Key to Media: minimal arabinose (MA), minimal glucose (MG), tetrazolium arabinose (TA). \ No newline at end of file |
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The Lenski Long-Term Evolution ExperimentArabinose Marker | ||||||||||
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The Lenski Long-Term Evolution ExperimentArabinose MarkerREL606 and REL607 (the ancestral strains of the E. coli long-term evolution experiment) differ by a single point mutation in the araA gene. This mutation was originally present in REL606. It makes an excellent neutral marker for evolution and competition experiments. REL607 was selected as a spontaneous arabinose-utilizing revertant from REL607. Most spontaneous revertants selected from a strain with the REL606 araA allele have the REL607 allele, but other mutations at this amino acid are also known to confer an Ara+ phenotype.
Summary of the Known Idiosyncrasies of Each Long-Term LineAfter more than 20 years and 40,000 generations of evolution in the laboratory, the various experimental replicates of the long-term experiment have diverged markedly. Some fail to grow on certain media.
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