Difference: EvolvabilityLongTermPlates (1 vs. 6)

Revision 62007-11-14 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Plate 1: Single Carbon Sources, Glucose and Glycerol Timescale

Changed:
<
<
1 1 D-Glucose 1
2 2 Glycerol 1
3 3 D-Ribose 1
4 4 Pyruvate 1
5 5 L-Malate 1
6 6 Acetate 1
>
>
1 1 D-Glucose
2 2 Glycerol
3 3 D-Ribose
4 4 Pyruvate
5 5 L-Malate
6 6 Acetate
 
7 2/1 Glycerol / D-Glucose, 1 transfer
8 2/1 Glycerol / D-Glucose, 2 transfers
9 2/1 Glycerol / D-Glucose, 4 transfers
10 2/1 Glycerol / D-Glucose, 8 transfers
11 2/1 Glycerol / D-Glucose, 16 transfers
12 2/1 Glycerol / D-Glucose, 32 transfers

Plate 2: Glucose and D-Ribose or Pyruvate Timescales

1 3/1 D-Ribose / D-Glucose, 1 transfer
2 3/1 D-Ribose / D-Glucose, 2 transfers
3 3/1 D-Ribose / D-Glucose, 4 transfers
4 3/1 D-Ribose / D-Glucose, 8 transfers
5 3/1 D-Ribose / D-Glucose, 16 transfers
6 3/1 D-Ribose / D-Glucose, 32 transfers
1 4/1 Pyruvate / D-Glucose, 1 transfer
2 4/1 Pyruvate / D-Glucose, 2 transfers
3 4/1 Pyruvate / D-Glucose, 4 transfers
4 4/1 Pyruvate / D-Glucose, 8 transfers
5 4/1 Pyruvate / D-Glucose, 16 transfers
6 4/1 Pyruvate / D-Glucose, 32 transfers

Plate 3: D-Glucose and L-Malate or Acetate Timescales

7 5/1 L-Malate / D-Glucose, 1 transfer
8 5/1 L-Malate / D-Glucose, 2 transfers
9 5/1 L-Malate / D-Glucose, 4 transfers
10 5/1 L-Malate / D-Glucose, 8 transfers
11 5/1 L-Malate / D-Glucose, 16 transfers
12 5/1 L-Malate / D-Glucose, 32 transfers
7 6/1 Acetate / D-Glucose, 1 transfer
8 6/1 Acetate / D-Glucose, 2 transfers
9 6/1 Acetate/ D-Glucose, 4 transfers
10 6/1 Acetate/ D-Glucose, 8 transfers
11 6/1 Acetate/ D-Glucose, 16 transfers
12 6/1 Acetate/ D-Glucose, 32 transfers

Plate 4: Other carbon compound pairs

7 3/2 D-Ribose / Glycerol, 8 transfers
8 4/2 Pyruvate/ Glycerol, 8 transfers
9 5/2 L-Malate / Glycerol, 8 transfers
10 6/2 Acetate/ Glycerol, 8 transfers
11 4/3 Pyruvate / D-Ribose, 8 transfers
12 5/3 Acetate / D-Ribose, 8 transfers
7 6/3 Acetate / D-Ribose, 8 transfer
8 5/4 Acetate / Pyruvate, 8 transfers
9 6/4 Acetate/ Pyruvate, 8 transfers
10 6/5 Acetate/ L-Malate, 8 transfers
11   Blanks
12    

Plate 1: 12 single carbon sources

General design considerations:

  1. Begin growth in substrate that supports lower cell density.

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose 1
2 2 D-Mannose 1?
3 3 D-Galactose 1
4 4 Glycerol 1
5 5 D-Ribose 2
6 6 β-Lactose 2
7 7 L-Rhamnose 4
8 8 L-Alanine 4
9 9 Pyruvate 4
10 10 D-Sorbitol 5
11 11 Acetate 8
12 12 L-Malic Acid 8

Plate 2: glucose / lactose scan + 5 carbon source pairs

1 6/1 β-Lactose / D-Glucose, 1 transfer
2 6/1 β-Lactose / D-Glucose, 2 transfers
3 6/1 β-Lactose / D-Glucose, 4 transfers
4 6/1 β-Lactose / D-Glucose, 8 transfers
5 6/1 β-Lactose / D-Glucose, 16 transfers
6 6/1 β-Lactose / D-Glucose, 32 transfers
7 6/1 β-Lactose / D-Glucose, 64 transfers
8 2/1 D-Mannose / D-Glucose, 8 transfers
9 3/1 D-Galactose / D-Glucose
10 4/1 Glycerol / D-Glucose
11 5/1 D-Ribose / D-Glucose
12 7/1 L-Rhamnose / D-Glucose

Plate 3: glucose / pyruvate scan + 5 carbon source pairs

1 9/1 Pyruvate / D-Glucose, 1 transfer
2 9/1 Pyruvate / D-Glucose, 2 transfers
3 9/1 Pyruvate / D-Glucose, 4 transfers
4 9/1 Pyruvate / D-Glucose, 8 transfers
5 9/1 Pyruvate / D-Glucose, 16 transfers
6 9/1 Pyruvate / D-Glucose, 32 transfers
7 9/1 Pyruvate / D-Glucose, 64 transfers
8 8/1 L-Alanine / D-Glucose, 8 transfers
9 10/1 D-Sorbitol / D-Glucose, 8 transfers
10 11/1 Acetate / D-Glucose, 8 transfers
11    
12    

2-4 2-5 2-6 2-7 2-10 2-11 3-5 3-6 3-7 3-9 3-10 4-7 4-8 4-9 4-10 5-6 5-10 5-11 5-12 6-8 6-12 7-8 7-9 7-10 7-12 8-11 8-12 9-10 10-11 10-12 11-12

Plate 4: glucose / L-malate scan + 5 carbon source pairs

1 12/1 D-Glucose / L-Malate, 1 transfer
2 12/1 D-Glucose / L-Malate, 2 transfers
3 12/1 D-Glucose / L-Malate, 4 transfers
4 12/1 D-Glucose / L-Malate, 8 transfers
5 12/1 D-Glucose / L-Malate, 16 transfers
6 12/1 D-Glucose / L-Malate, 32 transfers
7 12/1 D-Glucose / L-Malate, 64 transfers

Plate 5: 12 carbon source pairs

Plate 6: 12 carbon source pairs

First Generation Plates

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose
2 2 Glycerol
3 3 β-Lactose
4 4 D-Fructose
5 5 D-Ribose
6 6 D-Galactose
7 7 L-Rhamnose
8 8 Melibiose
9 9 D-Gluconic Acid
10 10 D-Sorbitol
11 11 Acetate
12 12 L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1 13 Pyruvic Acid
2 14 DL-Lactic Acid
3 17 Thymidine
4 18 Inosine
5 19 D-Trehalose
6 20 D-Serine
7 21 D-Glucuronic Acid
8 22 L-Alanine
9 23 D-Glucosamine
10 37 Glucuronamide
11 71 D-Arabinose
12 72 L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Proline, Thymidine, Acetate, D-Glucose 1
2 L-Proline, Thymidine, Acetate, D-Glucose 2
3 L-Proline, Thymidine, Acetate, D-Glucose 4
4 L-Proline, Thymidine, Acetate, D-Glucose 8
5 L-Proline, Thymidine, Acetate, D-Glucose 16
6 L-Proline, Thymidine, Acetate, D-Glucose 32
7 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 1
8 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 2
9 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 4
10 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 8
11 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 16
12 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 1
2 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 2
3 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 4
4 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 8
5 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 16
6 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 32
7 Acetate, D-Ribose, Glycerol, D-Glucose 1
8 Acetate, D-Ribose, Glycerol, D-Glucose 2
9 Acetate, D-Ribose, Glycerol, D-Glucose 4
10 Acetate, D-Ribose, Glycerol, D-Glucose 8
11 Acetate, D-Ribose, Glycerol, D-Glucose 16
12 Acetate, D-Ribose, Glycerol, D-Glucose 32

Revision 52007-11-14 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"
Changed:
<
<

Plate 1: 6 single carbon sources

>
>

Plate 1: Single Carbon Sources, Glucose and Glycerol Timescale

 
1 1 D-Glucose 1
2 2 Glycerol 1
3 3 D-Ribose 1
4 4 Pyruvate 1
Changed:
<
<
5 5 L-Malic Acid 1
>
>
5 5 L-Malate 1
 
6 6 Acetate 1
Added:
>
>
7 2/1 Glycerol / D-Glucose, 1 transfer
8 2/1 Glycerol / D-Glucose, 2 transfers
9 2/1 Glycerol / D-Glucose, 4 transfers
10 2/1 Glycerol / D-Glucose, 8 transfers
11 2/1 Glycerol / D-Glucose, 16 transfers
12 2/1 Glycerol / D-Glucose, 32 transfers

Plate 2: Glucose and D-Ribose or Pyruvate Timescales

1 3/1 D-Ribose / D-Glucose, 1 transfer
2 3/1 D-Ribose / D-Glucose, 2 transfers
3 3/1 D-Ribose / D-Glucose, 4 transfers
4 3/1 D-Ribose / D-Glucose, 8 transfers
5 3/1 D-Ribose / D-Glucose, 16 transfers
6 3/1 D-Ribose / D-Glucose, 32 transfers
1 4/1 Pyruvate / D-Glucose, 1 transfer
2 4/1 Pyruvate / D-Glucose, 2 transfers
3 4/1 Pyruvate / D-Glucose, 4 transfers
4 4/1 Pyruvate / D-Glucose, 8 transfers
5 4/1 Pyruvate / D-Glucose, 16 transfers
6 4/1 Pyruvate / D-Glucose, 32 transfers

Plate 3: D-Glucose and L-Malate or Acetate Timescales

7 5/1 L-Malate / D-Glucose, 1 transfer
8 5/1 L-Malate / D-Glucose, 2 transfers
9 5/1 L-Malate / D-Glucose, 4 transfers
10 5/1 L-Malate / D-Glucose, 8 transfers
11 5/1 L-Malate / D-Glucose, 16 transfers
12 5/1 L-Malate / D-Glucose, 32 transfers
7 6/1 Acetate / D-Glucose, 1 transfer
8 6/1 Acetate / D-Glucose, 2 transfers
9 6/1 Acetate/ D-Glucose, 4 transfers
10 6/1 Acetate/ D-Glucose, 8 transfers
11 6/1 Acetate/ D-Glucose, 16 transfers
12 6/1 Acetate/ D-Glucose, 32 transfers

Plate 4: Other carbon compound pairs

7 3/2 D-Ribose / Glycerol, 8 transfers
8 4/2 Pyruvate/ Glycerol, 8 transfers
9 5/2 L-Malate / Glycerol, 8 transfers
10 6/2 Acetate/ Glycerol, 8 transfers
11 4/3 Pyruvate / D-Ribose, 8 transfers
12 5/3 Acetate / D-Ribose, 8 transfers
7 6/3 Acetate / D-Ribose, 8 transfer
8 5/4 Acetate / Pyruvate, 8 transfers
9 6/4 Acetate/ Pyruvate, 8 transfers
10 6/5 Acetate/ L-Malate, 8 transfers
11   Blanks
12    
 

Plate 1: 12 single carbon sources

General design considerations:

  1. Begin growth in substrate that supports lower cell density.

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose 1
2 2 D-Mannose 1?
3 3 D-Galactose 1
4 4 Glycerol 1
5 5 D-Ribose 2
6 6 β-Lactose 2
7 7 L-Rhamnose 4
8 8 L-Alanine 4
9 9 Pyruvate 4
10 10 D-Sorbitol 5
11 11 Acetate 8
12 12 L-Malic Acid 8

Plate 2: glucose / lactose scan + 5 carbon source pairs

1 6/1 β-Lactose / D-Glucose, 1 transfer
2 6/1 β-Lactose / D-Glucose, 2 transfers
3 6/1 β-Lactose / D-Glucose, 4 transfers
4 6/1 β-Lactose / D-Glucose, 8 transfers
5 6/1 β-Lactose / D-Glucose, 16 transfers
6 6/1 β-Lactose / D-Glucose, 32 transfers
7 6/1 β-Lactose / D-Glucose, 64 transfers
8 2/1 D-Mannose / D-Glucose, 8 transfers
9 3/1 D-Galactose / D-Glucose
10 4/1 Glycerol / D-Glucose
11 5/1 D-Ribose / D-Glucose
12 7/1 L-Rhamnose / D-Glucose

Plate 3: glucose / pyruvate scan + 5 carbon source pairs

1 9/1 Pyruvate / D-Glucose, 1 transfer
2 9/1 Pyruvate / D-Glucose, 2 transfers
3 9/1 Pyruvate / D-Glucose, 4 transfers
4 9/1 Pyruvate / D-Glucose, 8 transfers
5 9/1 Pyruvate / D-Glucose, 16 transfers
6 9/1 Pyruvate / D-Glucose, 32 transfers
7 9/1 Pyruvate / D-Glucose, 64 transfers
8 8/1 L-Alanine / D-Glucose, 8 transfers
9 10/1 D-Sorbitol / D-Glucose, 8 transfers
10 11/1 Acetate / D-Glucose, 8 transfers
11    
12    

2-4 2-5 2-6 2-7 2-10 2-11 3-5 3-6 3-7 3-9 3-10 4-7 4-8 4-9 4-10 5-6 5-10 5-11 5-12 6-8 6-12 7-8 7-9 7-10 7-12 8-11 8-12 9-10 10-11 10-12 11-12

Plate 4: glucose / L-malate scan + 5 carbon source pairs

1 12/1 D-Glucose / L-Malate, 1 transfer
2 12/1 D-Glucose / L-Malate, 2 transfers
3 12/1 D-Glucose / L-Malate, 4 transfers
4 12/1 D-Glucose / L-Malate, 8 transfers
5 12/1 D-Glucose / L-Malate, 16 transfers
6 12/1 D-Glucose / L-Malate, 32 transfers
7 12/1 D-Glucose / L-Malate, 64 transfers

Plate 5: 12 carbon source pairs

Plate 6: 12 carbon source pairs

First Generation Plates

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose
2 2 Glycerol
3 3 β-Lactose
4 4 D-Fructose
5 5 D-Ribose
6 6 D-Galactose
7 7 L-Rhamnose
8 8 Melibiose
9 9 D-Gluconic Acid
10 10 D-Sorbitol
11 11 Acetate
12 12 L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1 13 Pyruvic Acid
2 14 DL-Lactic Acid
3 17 Thymidine
4 18 Inosine
5 19 D-Trehalose
6 20 D-Serine
7 21 D-Glucuronic Acid
8 22 L-Alanine
9 23 D-Glucosamine
10 37 Glucuronamide
11 71 D-Arabinose
12 72 L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Proline, Thymidine, Acetate, D-Glucose 1
2 L-Proline, Thymidine, Acetate, D-Glucose 2
3 L-Proline, Thymidine, Acetate, D-Glucose 4
4 L-Proline, Thymidine, Acetate, D-Glucose 8
5 L-Proline, Thymidine, Acetate, D-Glucose 16
6 L-Proline, Thymidine, Acetate, D-Glucose 32
7 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 1
8 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 2
9 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 4
10 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 8
11 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 16
12 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 1
2 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 2
3 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 4
4 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 8
5 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 16
6 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 32
7 Acetate, D-Ribose, Glycerol, D-Glucose 1
8 Acetate, D-Ribose, Glycerol, D-Glucose 2
9 Acetate, D-Ribose, Glycerol, D-Glucose 4
10 Acetate, D-Ribose, Glycerol, D-Glucose 8
11 Acetate, D-Ribose, Glycerol, D-Glucose 16
12 Acetate, D-Ribose, Glycerol, D-Glucose 32

Revision 42007-11-13 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"
Added:
>
>

Plate 1: 6 single carbon sources

1 1 D-Glucose 1
2 2 Glycerol 1
3 3 D-Ribose 1
4 4 Pyruvate 1
5 5 L-Malic Acid 1
6 6 Acetate 1
 

Plate 1: 12 single carbon sources

General design considerations:

  1. Begin growth in substrate that supports lower cell density.

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose 1
2 2 D-Mannose 1?
3 3 D-Galactose 1
4 4 Glycerol 1
5 5 D-Ribose 2
6 6 β-Lactose 2
7 7 L-Rhamnose 4
8 8 L-Alanine 4
9 9 Pyruvate 4
10 10 D-Sorbitol 5
11 11 Acetate 8
12 12 L-Malic Acid 8

Plate 2: glucose / lactose scan + 5 carbon source pairs

1 6/1 β-Lactose / D-Glucose, 1 transfer
2 6/1 β-Lactose / D-Glucose, 2 transfers
3 6/1 β-Lactose / D-Glucose, 4 transfers
4 6/1 β-Lactose / D-Glucose, 8 transfers
5 6/1 β-Lactose / D-Glucose, 16 transfers
6 6/1 β-Lactose / D-Glucose, 32 transfers
7 6/1 β-Lactose / D-Glucose, 64 transfers
8 2/1 D-Mannose / D-Glucose, 8 transfers
9 3/1 D-Galactose / D-Glucose
10 4/1 Glycerol / D-Glucose
11 5/1 D-Ribose / D-Glucose
12 7/1 L-Rhamnose / D-Glucose

Plate 3: glucose / pyruvate scan + 5 carbon source pairs

1 9/1 Pyruvate / D-Glucose, 1 transfer
2 9/1 Pyruvate / D-Glucose, 2 transfers
3 9/1 Pyruvate / D-Glucose, 4 transfers
4 9/1 Pyruvate / D-Glucose, 8 transfers
5 9/1 Pyruvate / D-Glucose, 16 transfers
6 9/1 Pyruvate / D-Glucose, 32 transfers
7 9/1 Pyruvate / D-Glucose, 64 transfers
8 8/1 L-Alanine / D-Glucose, 8 transfers
9 10/1 D-Sorbitol / D-Glucose, 8 transfers
10 11/1 Acetate / D-Glucose, 8 transfers
11    
12    

2-4 2-5 2-6 2-7 2-10 2-11 3-5 3-6 3-7 3-9 3-10 4-7 4-8 4-9 4-10 5-6 5-10 5-11 5-12 6-8 6-12 7-8 7-9 7-10 7-12 8-11 8-12 9-10 10-11 10-12 11-12

Plate 4: glucose / L-malate scan + 5 carbon source pairs

1 12/1 D-Glucose / L-Malate, 1 transfer
2 12/1 D-Glucose / L-Malate, 2 transfers
3 12/1 D-Glucose / L-Malate, 4 transfers
4 12/1 D-Glucose / L-Malate, 8 transfers
5 12/1 D-Glucose / L-Malate, 16 transfers
6 12/1 D-Glucose / L-Malate, 32 transfers
7 12/1 D-Glucose / L-Malate, 64 transfers

Plate 5: 12 carbon source pairs

Plate 6: 12 carbon source pairs

First Generation Plates

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose
2 2 Glycerol
3 3 β-Lactose
4 4 D-Fructose
5 5 D-Ribose
6 6 D-Galactose
7 7 L-Rhamnose
8 8 Melibiose
9 9 D-Gluconic Acid
10 10 D-Sorbitol
11 11 Acetate
12 12 L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1 13 Pyruvic Acid
2 14 DL-Lactic Acid
3 17 Thymidine
4 18 Inosine
5 19 D-Trehalose
6 20 D-Serine
7 21 D-Glucuronic Acid
8 22 L-Alanine
9 23 D-Glucosamine
10 37 Glucuronamide
11 71 D-Arabinose
12 72 L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Proline, Thymidine, Acetate, D-Glucose 1
2 L-Proline, Thymidine, Acetate, D-Glucose 2
3 L-Proline, Thymidine, Acetate, D-Glucose 4
4 L-Proline, Thymidine, Acetate, D-Glucose 8
5 L-Proline, Thymidine, Acetate, D-Glucose 16
6 L-Proline, Thymidine, Acetate, D-Glucose 32
7 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 1
8 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 2
9 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 4
10 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 8
11 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 16
12 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 1
2 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 2
3 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 4
4 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 8
5 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 16
6 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 32
7 Acetate, D-Ribose, Glycerol, D-Glucose 1
8 Acetate, D-Ribose, Glycerol, D-Glucose 2
9 Acetate, D-Ribose, Glycerol, D-Glucose 4
10 Acetate, D-Ribose, Glycerol, D-Glucose 8
11 Acetate, D-Ribose, Glycerol, D-Glucose 16
12 Acetate, D-Ribose, Glycerol, D-Glucose 32

Revision 32007-11-10 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Plate 1: 12 single carbon sources

Added:
>
>		General design considerations:
  1. Begin growth in substrate that supports lower cell density.
 Red Plate. Strains 606/607. 8 Replicates, one per column.
1 1 D-Glucose 1
2 2 D-Mannose 1?
3 3 D-Galactose 1
4 4 Glycerol 1
5 5 D-Ribose 2
6 6 β-Lactose 2
7 7 L-Rhamnose 4
8 8 L-Alanine 4
9 9 Pyruvate 4
10 10 D-Sorbitol 5
11 11 Acetate 8
12 12 L-Malic Acid 8
Changed:
<
<

Plate 2: 12 single carbon sources

>
>

Plate 2: glucose / lactose scan + 5 carbon source pairs

Added:
>
>
1 6/1 β-Lactose / D-Glucose, 1 transfer
2 6/1 β-Lactose / D-Glucose, 2 transfers
3 6/1 β-Lactose / D-Glucose, 4 transfers
4 6/1 β-Lactose / D-Glucose, 8 transfers
5 6/1 β-Lactose / D-Glucose, 16 transfers
6 6/1 β-Lactose / D-Glucose, 32 transfers
7 6/1 β-Lactose / D-Glucose, 64 transfers
8 2/1 D-Mannose / D-Glucose, 8 transfers
9 3/1 D-Galactose / D-Glucose
10 4/1 Glycerol / D-Glucose
11 5/1 D-Ribose / D-Glucose
12 7/1 L-Rhamnose / D-Glucose

Plate 3: glucose / pyruvate scan + 5 carbon source pairs

1 9/1 Pyruvate / D-Glucose, 1 transfer
2 9/1 Pyruvate / D-Glucose, 2 transfers
3 9/1 Pyruvate / D-Glucose, 4 transfers
4 9/1 Pyruvate / D-Glucose, 8 transfers
5 9/1 Pyruvate / D-Glucose, 16 transfers
6 9/1 Pyruvate / D-Glucose, 32 transfers
7 9/1 Pyruvate / D-Glucose, 64 transfers
8 8/1 L-Alanine / D-Glucose, 8 transfers
9 10/1 D-Sorbitol / D-Glucose, 8 transfers
10 11/1 Acetate / D-Glucose, 8 transfers
11    
12    

2-4 2-5 2-6 2-7 2-10 2-11 3-5 3-6 3-7 3-9 3-10 4-7 4-8 4-9 4-10 5-6 5-10 5-11 5-12 6-8 6-12 7-8 7-9 7-10 7-12 8-11 8-12 9-10 10-11 10-12 11-12

Plate 4: glucose / L-malate scan + 5 carbon source pairs

1 12/1 D-Glucose / L-Malate, 1 transfer
2 12/1 D-Glucose / L-Malate, 2 transfers
3 12/1 D-Glucose / L-Malate, 4 transfers
4 12/1 D-Glucose / L-Malate, 8 transfers
5 12/1 D-Glucose / L-Malate, 16 transfers
6 12/1 D-Glucose / L-Malate, 32 transfers
7 12/1 D-Glucose / L-Malate, 64 transfers

Plate 5: 12 carbon source pairs

Plate 6: 12 carbon source pairs

 

First Generation Plates

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose
2 2 Glycerol
3 3 β-Lactose
4 4 D-Fructose
5 5 D-Ribose
6 6 D-Galactose
7 7 L-Rhamnose
8 8 Melibiose
9 9 D-Gluconic Acid
10 10 D-Sorbitol
11 11 Acetate
12 12 L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1 13 Pyruvic Acid
2 14 DL-Lactic Acid
3 17 Thymidine
4 18 Inosine
5 19 D-Trehalose
6 20 D-Serine
7 21 D-Glucuronic Acid
8 22 L-Alanine
9 23 D-Glucosamine
10 37 Glucuronamide
11 71 D-Arabinose
12 72 L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Proline, Thymidine, Acetate, D-Glucose 1
2 L-Proline, Thymidine, Acetate, D-Glucose 2
3 L-Proline, Thymidine, Acetate, D-Glucose 4
4 L-Proline, Thymidine, Acetate, D-Glucose 8
5 L-Proline, Thymidine, Acetate, D-Glucose 16
6 L-Proline, Thymidine, Acetate, D-Glucose 32
7 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 1
8 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 2
9 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 4
10 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 8
11 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 16
12 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 1
2 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 2
3 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 4
4 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 8
5 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 16
6 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 32
7 Acetate, D-Ribose, Glycerol, D-Glucose 1
8 Acetate, D-Ribose, Glycerol, D-Glucose 2
9 Acetate, D-Ribose, Glycerol, D-Glucose 4
10 Acetate, D-Ribose, Glycerol, D-Glucose 8
11 Acetate, D-Ribose, Glycerol, D-Glucose 16
12 Acetate, D-Ribose, Glycerol, D-Glucose 32

Revision 22007-11-10 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"
Added:
>
>

Plate 1: 12 single carbon sources

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose 1
2 2 D-Mannose 1?
3 3 D-Galactose 1
4 4 Glycerol 1
5 5 D-Ribose 2
6 6 β-Lactose 2
7 7 L-Rhamnose 4
8 8 L-Alanine 4
9 9 Pyruvate 4
10 10 D-Sorbitol 5
11 11 Acetate 8
12 12 L-Malic Acid 8

Plate 2: 12 single carbon sources

First Generation Plates

 

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose
2 2 Glycerol
3 3 β-Lactose
4 4 D-Fructose
5 5 D-Ribose
6 6 D-Galactose
7 7 L-Rhamnose
8 8 Melibiose
9 9 D-Gluconic Acid
10 10 D-Sorbitol
11 11 Acetate
12 12 L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1 13 Pyruvic Acid
2 14 DL-Lactic Acid
3 17 Thymidine
4 18 Inosine
5 19 D-Trehalose
6 20 D-Serine
7 21 D-Glucuronic Acid
8 22 L-Alanine
9 23 D-Glucosamine
10 37 Glucuronamide
11 71 D-Arabinose
12 72 L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Proline, Thymidine, Acetate, D-Glucose 1
2 L-Proline, Thymidine, Acetate, D-Glucose 2
3 L-Proline, Thymidine, Acetate, D-Glucose 4
4 L-Proline, Thymidine, Acetate, D-Glucose 8
5 L-Proline, Thymidine, Acetate, D-Glucose 16
6 L-Proline, Thymidine, Acetate, D-Glucose 32
7 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 1
8 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 2
9 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 4
10 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 8
11 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 16
12 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 1
2 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 2
3 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 4
4 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 8
5 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 16
6 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 32
7 Acetate, D-Ribose, Glycerol, D-Glucose 1
8 Acetate, D-Ribose, Glycerol, D-Glucose 2
9 Acetate, D-Ribose, Glycerol, D-Glucose 4
10 Acetate, D-Ribose, Glycerol, D-Glucose 8
11 Acetate, D-Ribose, Glycerol, D-Glucose 16
12 Acetate, D-Ribose, Glycerol, D-Glucose 32

Revision 12007-11-02 - JeffreyBarrick

 
META TOPICPARENT name="ProtocolList"

Plate 1

Red Plate. Strains 606/607. 8 Replicates, one per column.

1 1 D-Glucose
2 2 Glycerol
3 3 β-Lactose
4 4 D-Fructose
5 5 D-Ribose
6 6 D-Galactose
7 7 L-Rhamnose
8 8 Melibiose
9 9 D-Gluconic Acid
10 10 D-Sorbitol
11 11 Acetate
12 12 L-Malic Acid

Plate 2

White Plate. Strains 606/607. 8 Replicates, one per column.

1 13 Pyruvic Acid
2 14 DL-Lactic Acid
3 17 Thymidine
4 18 Inosine
5 19 D-Trehalose
6 20 D-Serine
7 21 D-Glucuronic Acid
8 22 L-Alanine
9 23 D-Glucosamine
10 37 Glucuronamide
11 71 D-Arabinose
12 72 L-Proline

Plate 3

Red Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Proline, Thymidine, Acetate, D-Glucose 1
2 L-Proline, Thymidine, Acetate, D-Glucose 2
3 L-Proline, Thymidine, Acetate, D-Glucose 4
4 L-Proline, Thymidine, Acetate, D-Glucose 8
5 L-Proline, Thymidine, Acetate, D-Glucose 16
6 L-Proline, Thymidine, Acetate, D-Glucose 32
7 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 1
8 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 2
9 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 4
10 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 8
11 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 16
12 Pyruvic Acid, D-Galactose, β-Lactose, D-Glucose 32

Plate 4

White Plate. Strains 606/607. 8 Replicates, one per column.

  Treatment Transfers
1 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 1
2 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 2
3 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 4
4 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 8
5 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 16
6 L-Rhamnose, D-Galactose, D-Fructose, D-Glucose 32
7 Acetate, D-Ribose, Glycerol, D-Glucose 1
8 Acetate, D-Ribose, Glycerol, D-Glucose 2
9 Acetate, D-Ribose, Glycerol, D-Glucose 4
10 Acetate, D-Ribose, Glycerol, D-Glucose 8
11 Acetate, D-Ribose, Glycerol, D-Glucose 16
12 Acetate, D-Ribose, Glycerol, D-Glucose 32
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