Difference: DenaturingFormaldehydeGels (2 vs. 3)

Revision 32018-04-27 - SimonDAlton

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META TOPICPARENT name="ProtocolList"

Denaturing Formaldehyde Gels for Verifying RNA size

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  1. Add water to reach Xul total volume
  2. Add 3 volumes of NorthernMax buffer.
  3. The Millennium Markers need to be denatured also. Use 2ul + 6ul NorthernMax buffer.
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  1. Heat at 65C for 15 minutes. IMPORTANT When this has finished, immediately move samples to ice. If samples are allowed to slowly cool, some RNAs will bind to proteins and will get stuck in the wells of the gel.
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  1. Heat at 65C for 15 minutes. IMPORTANT When this has finished, immediately move samples to ice. If samples are allowed to slowly cool, some RNAs may bind to proteins/form secondary structure and will get stuck in the wells of the gel.
  RUNNING GEL
  1. Load samples as usual for a gel.
 
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