Assembly ReactionsUnder Construction Protocols from NEB BsaI and BsmBI toolkits All parts to be assembled must have correct recognition sites for BsaI or BsmBI, and correct 4bp overhangs to assemble in order. General Golden Gate Assembly protocol utilizing NEB Golden Gate toolkits (BsaI or BsmBI) In a PCR tube add:
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< < | Useful spreadsheet for calculating volumes of DNA to add GGA_Kit_Reaction_Calculation_Spreadsheet.xlsx | |||||||||||||||
> > | Useful spreadsheet for calculating volumes of DNA to add GGA_Kit_Reaction_Calculation_Spreadsheet0.xlsx | |||||||||||||||
Mix samples well by pipetting, then run the reaction on the thermocycler under the following conditions: For BsaI run at 37°C, for BsmBI use 42°C
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Assembly ReactionsUnder Construction Protocols from NEB BsaI and BsmBI toolkits All parts to be assembled must have correct recognition sites for BsaI or BsmBI, and correct 4bp overhangs to assemble in order. General Golden Gate Assembly protocol utilizing NEB Golden Gate toolkits (BsaI or BsmBI) In a PCR tube add: | ||||||||||||||||
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< < |
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< < | ||||||||||||||||
> > | Useful spreadsheet for calculating volumes of DNA to add GGA_Kit_Reaction_Calculation_Spreadsheet.xlsx | |||||||||||||||
Mix samples well by pipetting, then run the reaction on the thermocycler under the following conditions: For BsaI run at 37°C, for BsmBI use 42°C
![]() Comments |
Assembly ReactionsUnder Construction Protocols from NEB BsaI and BsmBI toolkits All parts to be assembled must have correct recognition sites for BsaI or BsmBI, and correct 4bp overhangs to assemble in order. General Golden Gate Assembly protocol utilizing NEB Golden Gate toolkits (BsaI or BsmBI) In a PCR tube add:
Mix samples well by pipetting, then run the reaction on the thermocycler under the following conditions: For BsaI run at 37°C, for BsmBI use 42°C
![]() Comments |