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| META TOPICPARENT |
name="ProtocolList" |
Generating Illumina Sequencing Libraries for transposons created in A. baylyi ADP1 with the pBT20 vector. |
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< | This protocol prepares Illumina sequencing libraries from Acinetobacter baylyi ADP1 mutagenized with the pBT20 conjugation vector. It is based on the tn-seq approach used by Eli Powell in the Moran lab in Snodgrassella alvi. Note: low retention eppindorf tubes and sterile filter tips should be used to avoid contaminating reagents and cross contaminating samples. |
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> | This protocol prepares Illumina sequencing libraries from Acinetobacter baylyi ADP1 mutagenized with the pBT20 conjugation vector. It is based on the tn-seq approach used by Eli Powell in the Moran lab in Snodgrassella alvi. Note: low retention eppindorf tubes and sterile filter tips should be used to avoid contaminating reagents and cross contaminating samples. |
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Uncommon lab materials needed before starting
- Covaris tubes for shearing
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- Go to step 2 19X
- Hold at 16°C
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< | Once complete, transfer the reaction to an eppindorf and remove the beads with a magnet. Clean up with 60 µL AMPure beads and elute in 25 µL dH2O. Qubit and submit a sample to the core for bioanayzer analysis. This can be done on a pooled library sample. |
>
> | Once complete, transfer the reaction to an Eppindorf and remove the beads with a magnet. Clean up with 60 µL AMPure beads and elute in 25 µL dH2O. Qubit and submit a sample to the core for bioanayzer analysis. This can be done on a pooled library sample. |
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-- Main.BrianRenda - 07 Apr 2016 |
