Please select a file to upload or enter a sequence into the textbox.

FASTA, GenBank, or BioBrick XML format files are supported. BioBrick XML files are available from the iGEM Registry.

Only assign rates for hypermutable sites that overlap with annotated regions. Sequence must be annotated.

Citing the EFM Calculator

Please reference the following publication when citing the EFM Calculator:

Benjamin R. Jack, Sean P. Leonard, Dennis M. Mishler, Brian A. Renda, Dacia Leon, Gabriel A. Suárez, and Jeffrey E Barrick (2015). Predicting the genetic stability of engineered DNA sequences with the EFM Calculator. ACS Synthetic Biology. Just Accepted Manuscript.
DOI: 10.1021/acssynbio.5b00068