Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	910,316	T→C	intergenic (+176/‑175)	ybjN/potF	predicted oxidoreductase/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	910,316	0	T→C	100.0%	119.7	38	intergenic (+176/‑175)	ybjN/potF	predicted oxidoreductase/putrescine transporter subunit: periplasmic‑binding component of ABC superfamily
Reads supporting (aligned to +/- strand):  new base (16/22):  ref base (0/0):  total (16/22)

TTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATG  >  REL606/910283‑910348                                  |                                 ttGTTAAATACCGTTTTTTAATCCGAGCTATAGcct                                <  1:4267571/36‑1 (MQ=255) ttGTTAAATACCGTTTTTTAATCCGAGCTATAGcct                                >  1:7259367/1‑36 (MQ=255)  ttttAAATACCGTTTTTTAATCCGAGCTATAGcctc                               <  1:2089873/34‑1 (MQ=37)  tGTTAAATACCGTTTTTTAATCCGAGCTATAGcctc                               >  1:3124858/1‑36 (MQ=255)  tGTTAAATACCCTTTTTTAATCCGAGCTATAGcctc                               >  1:5056182/1‑36 (MQ=25)   gTTAAATACCGTTTTTTAATCCGAGCTATAGCCTCa                              >  1:5726264/1‑36 (MQ=255)    ttAAATCCCGTTTTTTAATCGGAGCTATACCCTCaa                             <  1:6227955/36‑1 (MQ=18)      aaaTACCGTTTTTTAATCCGAGCTATAGCCTCAAAc                           >  1:6318814/1‑36 (MQ=255)      aaaTACCGTTTTTTAATCCGAGCTATAGCCTCAAAc                           <  1:3258890/36‑1 (MQ=255)       aaTACCGTTTTTTAATCCGAGCTATAGCCTCAAAcc                          >  1:4289298/1‑36 (MQ=255)        aTACCGTTTTTTAATCCGAGCTATAGCCTCAAAccc                         >  1:6918059/1‑36 (MQ=255)         tACCGTTTTTTAATCCGAGCTATAGCCTCAAACCCt                        >  1:3356150/1‑36 (MQ=255)          aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg                       <  1:4233186/36‑1 (MQ=255)          aCCGTTTTTTAATCCGAGCTATAGCCTCAAACCCTg                       <  1:3833585/36‑1 (MQ=255)           ccGTTTTTTAATCCGATCTATAGCCTCAAACCCTgg                      <  1:855243/36‑1 (MQ=25)             gTTTTTTAATCCGAGTTATAGCCTCAAACCCTGGCt                    <  1:370193/36‑1 (MQ=25)             gTTTTTAAATCCGAGCTATAGCCGCAAACCCTGGCt                    <  1:4786114/36‑1 (MQ=21)              ttttttAATCCGAGCTATAGCCTCAAACCCTGGCTa                   >  1:603025/1‑36 (MQ=255)               tttttAATCCGAGCTATAGCCTCAAACACTGGCTaa                  >  1:1078860/1‑36 (MQ=25)                ttttAGTCGGAGGTATAGCCTCAAACCCTGGCTaaa                 <  1:3118623/36‑1 (MQ=18)                ttttAATCCGAGCTATAGCCTCAAACCCTGGCTaaa                 <  1:2134799/36‑1 (MQ=255)                ttttAATCCGAGCTATAGCCTCAAACCCTGGCTaaa                 <  1:148075/36‑1 (MQ=255)                 tttAATCCGAGCTATAGCCTCAAACCCTGGCTAAAg                >  1:5619863/1‑36 (MQ=255)                 ttaaaTCCGAGCTATAGCCTCAAACCCTGGCTAAAg                <  1:4114610/33‑1 (MQ=25)                  ttAATCCGAGCTATAGCCTCAAACCCTGGCTAAAGt               >  1:2961037/1‑36 (MQ=255)                  ttAATCCGAGCTATAGCCTCAAACCCTGGCTAAAGt               <  1:6948607/36‑1 (MQ=255)                    aaTCCGAGCTATAGCCTCAAACCCTGGCTAAAGTTa             <  1:1825268/36‑1 (MQ=255)                     aTCGGAGCTATAGCCTCAAACCCTGGCTAAAGTTAt            <  1:5742235/36‑1 (MQ=25)                       ccGAGCTATAGCCTCAAACCCTGGCTAAAGTTATTc          >  1:5592720/1‑36 (MQ=255)                       ccGAGCTATAGCCTCAAACCCTGGCTAAAGTTATTc          >  1:3444113/1‑36 (MQ=255)                        cGAGCTTTAGCCTCAAACCCTGGCTAAAGTTATTCt         <  1:926813/36‑1 (MQ=25)                        cGAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCt         <  1:942100/36‑1 (MQ=255)                         gAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCtt        >  1:6124605/1‑36 (MQ=255)                         gAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCtt        >  1:306611/1‑36 (MQ=255)                         gAGCTATAGCCTCAAACCCTGGCTAAAGTTATTCtt        <  1:1064684/36‑1 (MQ=255)                           gCTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGc      <  1:786975/36‑1 (MQ=255)                           gCTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGc      >  1:2999361/1‑36 (MQ=255)                           gCTATAGCCCCAAACCCTGGCTAAAGTTATTCTTGc      <  1:986648/36‑1 (MQ=25)                            cTATAGCCTCAAACCCTGGCTAAAGTTATTCTTGCg     <  1:679623/36‑1 (MQ=255)                            cTATAGCCTCAAACCCTGGCTAAAGTTATTCTCGCg     >  1:4975314/1‑36 (MQ=25)                             tataGCCTCAAACCCTGGCTAAAGTTATTCTTGCGa    >  1:1082004/1‑36 (MQ=255)                              ataGCCTCAAACCCTGGCTAAAGTTATTCTTGCGAt   <  1:982278/36‑1 (MQ=255)                               taGCCTCAAACCCTGGCTAAAGTTATTCTTGCGATg  <  1:5140151/36‑1 (MQ=255)                                  |                                 TTGTTAAATACCGTTTTTTAATCCGAGCTATAGTCTCAAACCCTGGCTAAAGTTATTCTTGCGATG  >  REL606/910283‑910348 Base quality scores:  ATCG  < 3 ≤  ATCG  < 9 ≤  ATCG  < 17 ≤  ATCG  < 28 ≤  ATCG  < 32 ≤  ATCG