Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,616,396	G→A	G290S (GGC→AGC)	nadR	nicotinamide‑nucleotide adenylyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	4,616,396	0	G→A	100.0%	166.5	55	G290S (GGC→AGC)	nadR	nicotinamide‑nucleotide adenylyltransferase
Reads supporting (aligned to +/- strand):  new base (34/19):  ref base (1/1):  total (35/20)

TTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAA  >  REL606/4616363‑4616428                                  |                                 ttGCAGTATTCCGATTACGATAAAATCGCGCTGAGc                                >  1:4053025/1‑36 (MQ=255)   gCAGTATTCCGATTACGATAAAATCGCGCTGAGcaa                              >  1:7000973/1‑34 (MQ=25)   gCAGTATTCCGATTACGATAAAATCGCGCTGAGCCa                              <  1:1728794/36‑1 (MQ=255)   gCAGTATTCCGATTACGATAAAATCGCGCTGAGCCa                              >  1:1735257/1‑36 (MQ=255)   gCAGTATTCCGATTACGATAAAATCGCGCTGAGCCa                              >  1:7001514/1‑36 (MQ=255)   gCAGTATTCCGATTACGATAAAATCGCGCTGAGCCa                              <  1:698032/36‑1 (MQ=255)     aGTATTCCGATTACGATAAAATCGCGCTGAGCCACg                            >  1:5071174/1‑36 (MQ=255)      gTATTCCGATTACGATAAAATCGCGCTGAGCCACGc                           <  1:3760760/36‑1 (MQ=255)      gTATTCCGATTACGATAAAATCGCGCTGAGCCACGc                           <  1:1561291/36‑1 (MQ=255)        aTTCCGATTACGATAAAATCGCGCTGAGCCACGcac                         >  1:5750874/1‑36 (MQ=255)          tCCGATTACGATAAAATCGCGCTGAGCCACGCACaa                       <  1:1924497/36‑1 (MQ=255)           ccGATTACGATAAAATCGCGCTGAGCCACGCACAAt                      <  1:1448935/36‑1 (MQ=255)           ccGATTACGATAAAATCGCGCTGAGCCACCCACAAt                      >  1:4407146/1‑36 (MQ=25)            cGATTACGATAAAATCGCGCTGAGCCACGCACAATa                     <  1:2083970/36‑1 (MQ=255)             gATTACGATAAAATCGCGCTGAGCCACGCACAATAc                    <  1:3696316/36‑1 (MQ=255)              aTTACGATAAAATCGCGCTGAGCCACGCACAATACa                   <  1:2265582/36‑1 (MQ=255)              aTTACGATAAAATCGCGCTGAGCCACGCACAATACa                   >  1:140752/1‑36 (MQ=255)              aTTACGATAAAATCGCGCTGAGCCACGCACAATACa                   <  1:1167370/36‑1 (MQ=255)               ttACGATAAAATCGCGCTGAGCCACGCATAATACAt                  >  1:4530314/1‑36 (MQ=25)               ttACGATAAAATCGCGCTGAGCCACGCACAATACAt                  <  1:6632169/36‑1 (MQ=255)               ttACGATAAAATCGCGCTGAGCCACGCACAATACAt                  >  1:3720805/1‑36 (MQ=255)                tACGATAAAATCGCGCTGGGCCACGCACAATACAtt                 >  1:7251599/1‑36 (MQ=255)                tACGATAAAATCGCGCTGAGCCACGCACAATACAtt                 <  1:186046/36‑1 (MQ=255)                 aCGATAAAATCGCGCTGAGCTACGCACAATACATTg                >  1:987676/1‑36 (MQ=25)                 aCGATAAAATCGCGCTGAGCCACGCACAATACATTg                <  1:6552078/36‑1 (MQ=255)                 aCGATAAAATCGCGCTGAGCCACGCACAATACATTg                >  1:5541506/1‑36 (MQ=255)                 aCGATAAAATCGCGCTGAGCCACGCACAATACATTg                <  1:990717/36‑1 (MQ=255)                 aCGATAAAATCGCGCTGAGCCACGCACAATACATTg                >  1:1947677/1‑36 (MQ=255)                  cGATAAAATCGCGCTGAGCCACGCACAATACATTGa               <  1:6523659/36‑1 (MQ=255)                   gATAAAATCGCGCTGAGCCACGCACAATACATTGAt              <  1:6795178/36‑1 (MQ=255)                   gATAAAATCGCGCTGAGCCACGCACAATACATTGAt              >  1:229018/1‑36 (MQ=255)                   gATAAAATCGCGCTGAGCCACGCACAATACATTGAt              >  1:3342035/1‑36 (MQ=255)                    aTAAAATCGCGCTGAGCCACGCACAATACATTGAtt             >  1:115898/1‑36 (MQ=255)                    aTAAAATCGCGCTGAGCCACGCACAATACATTGAtt             >  1:516929/1‑36 (MQ=255)                     tAAAATCGCGCTGAGCCACGCACCATACATTGAttt            >  1:6712816/1‑36 (MQ=25)                     tAAAATCGCGCTGAGCCACGCACAATACATTGAttt            >  1:6286351/1‑36 (MQ=255)                     tAAAATCGCGCTGAGCCACGCACAATACATTGAttt            >  1:6087213/1‑36 (MQ=255)                      aaaaTCGCGCTGAGCCACGCACAATACATTGAtttt           >  1:4134416/1‑36 (MQ=255)                      aaaaTCGCGCTGAGCCACGCACAATACATTGAtttt           >  1:210263/1‑36 (MQ=255)                       aaaTCGCGCTGAGCCACGCACAATACATTGATTTTg          >  1:4823627/1‑36 (MQ=255)                        aaTCGCGCTGAGCCACGCACAATACATTGTTTTTgt         >  1:736659/1‑35 (MQ=25)                         aTCGCGCTGAGCCACGCACAATACATTGATTTTGCa        <  1:1777168/36‑1 (MQ=255)                         aTCGCGCTGAGCCACGCACAATACATTGATTTTGCa        >  1:1623730/1‑36 (MQ=255)                          tCGCGCTAAGCCACGCACAATACATTGATTTTGCAg       <  1:3197348/36‑1 (MQ=25)                           cgcgCTGGGCCACGCGCAATACATTGATTTTGCAGt      <  1:7349442/36‑1 (MQ=255)                           cgcgCTGAGCCACGCACAATACATTGATTTTGCAGt      >  1:3847110/1‑36 (MQ=255)                           cgcgCGGAGCCACGCACAATACATTGATTTTGCAGt      <  1:3847385/36‑1 (MQ=25)                            gcgcTGAGCCACGCACAATACATTGATTTTGCTGTg     >  1:5278341/1‑36 (MQ=25)                            gcgcTGAGCCACGCACAATACATTGATTTTGCTGTg     >  1:4003210/1‑36 (MQ=25)                             cgcTGAGCCACGCACAATACATTGATTTTGCAGTGa    >  1:3196539/1‑36 (MQ=255)                             cgcTGAGCCACGCACAATACATTGATTTTGAAGTGa    >  1:5693924/1‑36 (MQ=25)                              gcTGAGCCACGCCCAATACATTGATTTTGCAGTGaa   >  1:4651127/1‑36 (MQ=25)                              gcTGAGCCACGCACAATACATTGATTTTGCAGTGaa   >  1:972411/1‑36 (MQ=255)                              gcTGAGCCACGCACAATACATTCATTCTGCAGTGaa   >  1:1734716/1‑36 (MQ=21)                               cTGAGCCACGCACAATACATTGATTTTGCAGCGaaa  >  1:2734989/1‑36 (MQ=25)                                  |                                 TTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAA  >  REL606/4616363‑4616428 Base quality scores:  ATCG  < 3 ≤  ATCG  < 11 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG