Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,100,655	C→T	M192I (ATG→ATA)	hslU	ATP‑dependent protease ATP‑binding subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	4,100,655	0	C→T	100.0%	114.1	37	M192I (ATG→ATA)	hslU	ATP‑dependent protease ATP‑binding subunit
Reads supporting (aligned to +/- strand):  new base (26/11):  ref base (0/0):  total (26/11)

GAACATGGACTGCAGCTGGCTGGTCATCTCTTCCATGCCCGGAGGAGCCATAATTTCAACGCCCAT  >  REL606/4100622‑4100687                                  |                                 gAACATGGACTGCAGCTGGCTGGTCATGTCTTCTAt                                >  1:6835466/1‑36 (MQ=25) gAACATGGACTGCAGCTGGCTGGTCATCTCTTCTAt                                >  1:1662551/1‑36 (MQ=255) gAACAGGGGCTGCAGCTGGCTGGTCATCTCTTCTAt                                <  1:3340617/36‑1 (MQ=21)  aaCATGGACTGCAGCTGGCTGGTCATCTCTTCTATg                               >  1:3380696/1‑36 (MQ=255)    cATGGACTGCAGCTGGCTGGTCATCTCTTCTATGcc                             >  1:3131550/1‑36 (MQ=255)    cATGGACTGCAGCTAGCTGGTCATCTCTTCTATGcc                             >  1:5321122/1‑36 (MQ=25)     aTGGACTGCAGCTGGCTGGTCATCTCTTCTATGccc                            >  1:1324348/1‑36 (MQ=255)      tGGACTGCAGCTGGCTGGTCATCTCTTCTATGCCCg                           <  1:5750787/36‑1 (MQ=255)       ggACTGCAGCTGGCTGGTCATCTCTTCTATGCCCgg                          >  1:6164893/1‑36 (MQ=255)       ggACTGCAGCTGGCTGGTCATCTCTTCTACGCCCgg                          >  1:7453117/1‑36 (MQ=25)        gACTGCAGCTGGCTGGTCATCTCTTCTATGCCCgga                         >  1:5317502/1‑36 (MQ=255)         aCTGCAGCTGGCTGGTCCTCTCTTCTATGCCCggag                        >  1:4477820/1‑36 (MQ=25)         aCTGCAGCTGGCTGGTCATCTCTTCTATGCCCggag                        <  1:5056169/36‑1 (MQ=255)          cTGCAGCTGGCTGGTCATCTCTTCTATGCCCggagg                       >  1:7304054/1‑36 (MQ=255)          cTGCAGCTGGCTGGTCATCTCTTCTATGCCCggagg                       <  1:6516980/36‑1 (MQ=255)          cTGCAGCTGGCTGGTCATCTCTTCTATGCCCggaag                       >  1:2735439/1‑34 (MQ=25)           tGCAGCTGGCTGGTCATCTCTTCTATGCCCggagga                      >  1:7132063/1‑36 (MQ=255)           tGCAGCTGGCTGGTCATCTCTTCTATGCCCggagga                      <  1:6758273/36‑1 (MQ=255)            gCAGCTGGCTGGTCATCTCTTCTATGCCCGgaggag                     <  1:3845660/36‑1 (MQ=255)              aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc                   <  1:4568340/36‑1 (MQ=255)              aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc                   >  1:4183666/1‑36 (MQ=255)              aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc                   <  1:3661005/36‑1 (MQ=255)              aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc                   >  1:2266289/1‑36 (MQ=255)              aGCTGGCTGGTCATCTCTTCTATGCCCGGAGGAGcc                   >  1:1028490/1‑36 (MQ=255)              aGCTGGCTGGTCATCTCTTCTACGCCCGGAGCAGcc                   >  1:1735116/1‑36 (MQ=21)               gctggttgGTCATCTCTTCTATGCCCGGAGGAGCCa                  >  1:400876/1‑36 (MQ=25)               gctggctgGTCATCTCTTCTATGCCCGGAGGAGCCa                  <  1:7040167/36‑1 (MQ=255)                 tggctggTCATCTCTTGTATGCCGGGAGGAGCCATa                <  1:82341/36‑1 (MQ=21)                    ctggTCATCTCTTCTATGCCCGGAGGTGCCATAAtt             >  1:6601466/1‑36 (MQ=25)                     ttttCATCTCTTCTATTCCGGGAGGAGCCATAAttt            <  1:428870/33‑1 (MQ=18)                     tggTCATCTCTTCTATGCCCGGAGGAGCCATAAttt            >  1:6388963/1‑36 (MQ=255)                      ggTCATCTCTTCTATGCCCGGAGGAGCCATAATTTc           >  1:4146208/1‑36 (MQ=255)                       gTCATCTCTTCTATGCCCGGAGGAGCCATAATTTCa          >  1:3230597/1‑36 (MQ=255)                        tCATCTCTTCTATGCCCGGAGGAGCCATAATTTCaa         >  1:2030543/1‑36 (MQ=255)                           tctcTTCTATGCCCGGAGGAGCCATAATTTCAACGc      >  1:6024163/1‑36 (MQ=255)                              ctTCTATGCCCGGAGGAGCCATAATTTCAACGCCCa   >  1:2668308/1‑36 (MQ=255)                               ttCTATGCCCGGAGGAGCCATAATTTCAACGCCCAt  >  1:4114639/1‑36 (MQ=255)                                  |                                 GAACATGGACTGCAGCTGGCTGGTCATCTCTTCCATGCCCGGAGGAGCCATAATTTCAACGCCCAT  >  REL606/4100622‑4100687 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 16 ≤  ATCG  < 29 ≤  ATCG  < 32 ≤  ATCG