Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,369,432	T→C	D50G (GAC→GGC)	rpsD	30S ribosomal protein S4

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	3,369,432	0	T→C	100.0%	206.4	63	D50G (GAC→GGC)	rpsD	30S ribosomal protein S4
Reads supporting (aligned to +/- strand):  new base (44/18):  ref base (0/0):  total (44/19)

CTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGG  >  REL606/3369400‑3369464                                 |                                 cTTTTTGCTTTTCACGCAACTGCACACCATAGCcag                               <  1:3734486/36‑1 (MQ=255) cTTTTTGCTTTTCACGCAACTGCACACCATAGCcag                               >  1:2827041/1‑36 (MQ=255) cTTTTTGCTTTTCACCCAACTGCACACCATAGCcag                               <  1:6554935/36‑1 (MQ=25)   ttttGCTTTTCACGCAACTGCACACCATAGCcagtc                             >  1:1855894/1‑34 (MQ=25)   ttttGCTTTTCACGCAACTGCACACCATAGCcagac                             >  1:3167795/1‑36 (MQ=255)    tttGCTTTTCACGCAACTGCACACCATAGCcagaca                            >  1:1450586/1‑36 (MQ=255)     ttGCTTTTCACGCAACTGCACACCATAGCcagacag                           >  1:7415941/1‑36 (MQ=255)     ttGCTTTTCACGCAACTGCACACCATAGCcagacag                           >  1:369809/1‑36 (MQ=255)     ttGCTTTTCACGCAACTGCACACCATAGCcagacag                           >  1:3355888/1‑36 (MQ=255)      tGCTTTTCACGCAACTGCACACCATAGCcagacaga                          <  1:5926815/36‑1 (MQ=255)       gCTTTTCACGCAACTGCACACCATAGCCagacagac                         >  1:494902/1‑36 (MQ=255)       gCTTTTCACGCAACTGCACACCATAGCCagacagac                         <  1:5834242/36‑1 (MQ=255)       gCTTTTCACGCAACTGCACACCATAGCCagacagac                         <  1:7439414/36‑1 (MQ=255)        cTTTTCACGCAACTGCACACCATAGCCAGACAGAcg                        >  1:5515402/1‑36 (MQ=255)        cTTTTCACGCAACTGCACACCATAGCCAGACAGAcg                        >  1:2936609/1‑36 (MQ=255)         ttttCACGCAACTGCACACCATACCCACACACAcgc                       >  1:2886382/1‑36 (MQ=18)          tttCACGCAACTGCACACCATAGCCAGACAGAcgcg                      >  1:1555998/1‑36 (MQ=255)          tttCACGCAACTGCACACCATAGCCAGACAGAcgcg                      >  1:2133170/1‑36 (MQ=255)           ttCACGCAACTGCACACCATAGCCAGACAGACGCgg                     >  1:4876034/1‑36 (MQ=255)            tCACGCAACTGCACACCATAGCCAGACAGAcgcgct                    >  1:1899743/1‑34 (MQ=25)            tCACGCAACTGCACACCATAGCCAGACAGACGCGGt                    >  1:4807284/1‑36 (MQ=255)             cACGCAACTGCACACCATAGCCAGACAGACGAGGtt                   >  1:6766915/1‑36 (MQ=25)              aCGCAACTGCACACCATAGCCAGACAGACGCGGttt                  <  1:5009754/36‑1 (MQ=255)              aCGCAACTGCACACCATAGCCAGACAGACGCGGttt                  <  1:5529244/36‑1 (MQ=255)              aCGCAACTGCACACCATAGCCAGACAGACGCGGttt                  >  1:3360607/1‑36 (MQ=255)              aCGCAACTGCACACCATAGCCAGACAGACGCGGttt                  >  1:858144/1‑36 (MQ=255)               cGCAACTGCACACCATAGCCAGACAGACGCGGTTTa                 >  1:4651140/1‑36 (MQ=255)                gCAACTGCACACCATAGCCAGACAGACGCGGTTTAc                >  1:1970939/1‑36 (MQ=255)                gCAACTGCACACCATAGCCAGACAGACGCGGTTTAc                >  1:1979882/1‑36 (MQ=255)                aaaaCTACACACCATAGCCAGACAGACGCGGTTTAc                <  1:1141489/34‑1 (MQ=25)                 cAACTGCACACCATAGCCAGACAGACGCGGTTTAcg               >  1:1604366/1‑36 (MQ=255)                 cAACTGCACACCATAGCCAGACAGACGCGGTTTAcg               <  1:2415627/36‑1 (MQ=255)                  aaCTGCACACCATAGCCAGACAGACGCGGTTTAcgc              >  1:5501233/1‑36 (MQ=255)                  aaCTGCACACCATAGCCAGACAGACGCGGTTTAcgc              >  1:2876524/1‑36 (MQ=255)                  aaCTGCACACCATAGCCAGACAGACGCGGTTTAcgc              <  1:4563514/36‑1 (MQ=255)                   aCTGCACAGCATAGCCGGACAGACGCGGTTTAcgcg             <  1:2549300/36‑1 (MQ=21)                   aCTGCACACCATTGCCAGTCAGACGCGGTTTAcgcg             >  1:4363932/1‑36 (MQ=21)                   aCTGCACACCATAGCCAGACAGACGCGGTTTAcgcg             >  1:374614/1‑36 (MQ=255)                   aCTGCACACCATAGCCAGACAGACGCGGTTTAcgcg             >  1:5086901/1‑36 (MQ=255)                    cTGCACACCATAGCCAGACAGACGCGGTTTACgcgc            >  1:5469750/1‑36 (MQ=255)                    cTGCACACCATAGCCAGACAGACGCGGTTTACgcgc            >  1:5981601/1‑36 (MQ=255)                    cTGCACACCATAGCCAGACAGACGCGGTTTACgcgc            <  1:2973007/36‑1 (MQ=255)                    cTGCACACCATAGCCAGACAGACGCGGTTTACgcgc            >  1:7325542/1‑36 (MQ=255)                    cTGCACACCATAGCCAGACAGACGCGGTTTACgcgc            >  1:1668126/1‑36 (MQ=255)                    cTGCACACCATAGCCAGACAGACGCGGTTTACacgc            >  1:4356502/1‑36 (MQ=25)                     tGCACACCATAGCCAGACAGACGCGGTTTTCGCGCa           >  1:1120981/1‑36 (MQ=21)                     tGCACACCATAGCCAGACAGACGCGGTTTACGCGCa           >  1:7378104/1‑36 (MQ=255)                     tGCACACCATAGCCAGACAGACGCGGTTTACGCGCa           >  1:3825907/1‑36 (MQ=255)                     tGCACACCATAGCCAGACAGACGCGGTTTACACGCa           >  1:2239932/1‑36 (MQ=25)                      gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc          >  1:122656/1‑36 (MQ=255)                      gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc          <  1:2633350/36‑1 (MQ=255)                      gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc          <  1:3744109/36‑1 (MQ=255)                      gCACACCATAGCCAGACAGACGCGGTTTACGCGCAc          <  1:5197057/36‑1 (MQ=255)                       cacaCCATAGCCAGACAGACGCGGTTTATGCGTAca         >  1:6442356/1‑35 (MQ=21)                        acacCATAGCCAGACAGACGCGGTTTACGCGCACCg        <  1:7265844/36‑1 (MQ=255)                        acacCAGAGCCAGACAGACGCGGTTTACGCGCACCg        <  1:42763/36‑1 (MQ=21)                         cacCATAGCCAGACAGACGCGGTTTACGCGCACCGt       <  1:5674173/36‑1 (MQ=255)                          acCATAGCCAGACAGACGCGGTTTACGCGCACCGTg      >  1:6784021/1‑36 (MQ=255)                           ccATAGCCAGACAGACGCGGTTTACGCGCACCGTGc     >  1:3737860/1‑36 (MQ=255)                           ccATAGCCAGACAGACGCGGTTTACGCGCACCGTGc     >  1:2280308/1‑36 (MQ=255)                           ccATAGCCAGACAGACGCGGTTTACGCGCACCGTGc     >  1:1501667/1‑36 (MQ=255)                            cATAGCCAGACAGACGCGGTTTACGCGCACCGTGCt    >  1:5166114/1‑36 (MQ=255)                              tAGACAGACAGACGCGGTTTACGCGCACCGTGCTgg  <  1:6205993/36‑1 (MQ=255)                                 |                                 CTTTTTGCTTTTCACGCAACTGCACACCATAGTCAGACAGACGCGGTTTACGCGCACCGTGCTGG  >  REL606/3369400‑3369464 Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 19 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG