Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,732,014	C→A	R91L (CGC→CTC)	hypF	carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	2,732,014	0	C→A	100.0%	122.7	39	R91L (CGC→CTC)	hypF	carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  new base (17/22):  ref base (0/0):  total (17/22)

TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGGATAGTGAACTCAGTGGGCAGTTGTGACCAG  >  REL606/2731982‑2732046                                 |                                 ttggCGTATTCATGGTGCCGCCTGTGCTCTGGAGGa                               <  1:898426/33‑1 (MQ=25)  ttGCGTATTCGTGGTGCCGCCTGTGCTCTGGAGGAt                              <  1:5185498/36‑1 (MQ=25)  ttGCGTATTCATGGTGCCGCCTGTGCTCTGGAGGAt                              <  1:439238/36‑1 (MQ=255)  ttGCGTATTCATGGTGCCGCCTGTGCTCTGGAGGAt                              >  1:5314835/1‑36 (MQ=255)   tGCGTATTCATGGTGCCGCCTGTGCTCTGGAGGATa                             <  1:5260053/36‑1 (MQ=255)     cGTATTCATGGTGCCGCCTGTGCTCTGGAGGATAGt                           >  1:1831413/1‑36 (MQ=255)       tATTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGa                         <  1:4812545/36‑1 (MQ=255)       tATTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGa                         >  1:330075/1‑36 (MQ=255)        aTTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGaa                        >  1:1597807/1‑36 (MQ=255)        aTTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGaa                        >  1:2666628/1‑36 (MQ=255)        aTTCATGGTGCCGCCTGTGCTCTGGAGGATAGTGaa                        <  1:354875/36‑1 (MQ=255)           cATGGTGCCGCCTGTGCTCTGGAGGATAGTGAACTc                     <  1:7341578/36‑1 (MQ=255)           cATGGTGCCGCCTGTGCTCTGGAGGATAGTGAACTc                     >  1:1737417/1‑36 (MQ=255)           cATGGTGCCGCCTGTGCTCTGGAGGATAGTGAACTc                     >  1:1215684/1‑36 (MQ=255)            aTGGTGCCGCCTGTGCTCTGGAGGATAGTGAACTCa                    >  1:4927001/1‑36 (MQ=255)            aTGGTGCCGCCTGTGCTCTGGAGGATAGTGAACTCa                    <  1:5799695/36‑1 (MQ=255)            aTGGTCCCGCCTGTGCTCTGGAGGATAGTGAACTCa                    <  1:5634224/36‑1 (MQ=25)              ggTGCCGCCTGTGCTCTGGAGGATAGTGAACTCAGt                  <  1:1187813/36‑1 (MQ=255)              aatGCCACCTATACTCTGAAGGATAGTGAACTCAGt                  <  1:956353/34‑1 (MQ=12)                ggccgccTGTGCTCTGGAGGATAGTGAACTCAGTgg                <  1:507396/35‑1 (MQ=37)                 gccgccTGTGCTCTGGAGGATAGTGAACTCAGTggg               >  1:1096182/1‑36 (MQ=255)                  ccggcTGTGCTCCGGAGGATAGTGAACTCAGTGGGc              <  1:4780856/36‑1 (MQ=21)                    gccTGTGCTCTGGAGGATAGTGAACTCAGTGGGCAg            >  1:4746820/1‑36 (MQ=255)                    gccTGTGCTCTGGAGGATAGTGAACTCAGTGGGCAg            >  1:7485611/1‑36 (MQ=255)                      cTGTTCTCTGGAGTATAGTGAACTCAGTGGGCAGtt          <  1:1651585/36‑1 (MQ=21)                      cTGTGCTCTGGAGGATAGTGAACTCAGTGGGCAGtt          >  1:2249605/1‑36 (MQ=255)                       tgtgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtg         >  1:2278198/1‑36 (MQ=255)                        gtgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgt        <  1:948204/36‑1 (MQ=255)                        gtgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgt        <  1:5947889/36‑1 (MQ=255)                        gtgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgt        <  1:5324286/36‑1 (MQ=255)                        gtgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgt        <  1:4891124/36‑1 (MQ=255)                        gtgCTCTGGAGGATAGTGAACTCAGTGGGCAGCtgt        >  1:5642337/1‑36 (MQ=25)                         tgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgtg       <  1:6485189/36‑1 (MQ=255)                         tgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgtg       <  1:1806980/36‑1 (MQ=255)                         tgCTCTGGAGGATAGTGAACTCAGTGGGCAGTtgtg       >  1:1088202/1‑36 (MQ=255)                           ctctGGAGGATAGTGAACTCAGTGGGCAGTTGTGAc     >  1:20411/1‑36 (MQ=255)                              tGGAGGATAGTGAACTCAGTGGGCAGTTGTGATCAg  >  1:5200831/1‑36 (MQ=25)                              tGGAGGATAGTGAACTCAGTGGGCAGTTGTGACCAg  <  1:5438168/36‑1 (MQ=255)                              tGGAGGATAGTGAACTCAGTGGGCAGTTGTGACCAg  <  1:865187/36‑1 (MQ=255)                                 |                                 TTTGCGTATTCATGGTGCCGCCTGTGCTCTGGCGGATAGTGAACTCAGTGGGCAGTTGTGACCAG  >  REL606/2731982‑2732046 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 32 ≤  ATCG