Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,248,380	A→C	D295E (GAT→GAG)	dhaM	fused predicted dihydroxyacetone‑specific PTS enzymes: HPr component/EI component

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	1,248,380	0	A→C	100.0%	92.8	33	D295E (GAT→GAG)	dhaM	fused predicted dihydroxyacetone‑specific PTS enzymes: HPr component/EI component
Reads supporting (aligned to +/- strand):  new base (15/16):  ref base (0/0):  total (17/16)

CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT  >  REL606/1248348‑1248410                                 |                               cTGGCTTCTGCTTTCGCTGTTAACGTCATCAGCTCt                             <  1:5565933/36‑1 (MQ=255) cTGGCTTCTGCTTTCGCTGTTAACGTCATCAGCTCt                             >  1:490537/1‑36 (MQ=255)  tGGCTTCTGCTTTCGCTGTTAACGTCATCAGCTCta                            >  1:5306964/1‑36 (MQ=255)   ggCTTCTGCTTTCGCTGTTAACGTCATCAGCTCtaa                           >  1:2731878/1‑36 (MQ=255)    gCTTCTGCTTTCGCTGTTAACGTCATCAGTTCtaat                          >  1:3730187/1‑36 (MQ=255)     cTTCTGCTTTCGCTGTTAACGTCATCAGCTCtaata                         <  1:6225216/36‑1 (MQ=255)     cTTCTGCTTTCGCTGTTAACGTCATCAGCTCtaata                         >  1:7163586/1‑36 (MQ=255)      ttCTGCTTTCGCTGTTAACGTCATCAGCTCtaataa                        >  1:3427656/1‑36 (MQ=255)      ttCTGCTTTCGCTGTTAACGTCATCAGCTCtaataa                        <  1:1930320/36‑1 (MQ=255)      ttCTGCTTTCGCTGTTAACGTCATCAGCTCtaataa                        >  1:1717733/1‑36 (MQ=255)       tCTGCTTTCGCTGTTAACGTCATCAGCTCTAATAAc                       <  1:5103847/36‑1 (MQ=255)        cTGCTTTCGCTGTTAACGTCATCAGTTCtaataatg                      >  1:590347/1‑34 (MQ=25)        cTGCTTTCGCTGTTAACGTCATCAGCTCTAACAACg                      >  1:2767545/1‑36 (MQ=25)        cTGCTTTAGATGTTAACGTCATCAGCTCTAATAACg                      <  1:237367/36‑1 (MQ=21)         ttcTTTCGCTGTTAACGTCATCAGCTCTAATAACGt                     <  1:390440/34‑1 (MQ=25)          gCTTTCGCTGTTAACGTCATCAGCTCTAATAACGTg                    <  1:1874264/36‑1 (MQ=255)           cTTTCGCTGTTAACGTCATCAGCTCTAATAACGTGa                   >  1:2426042/1‑36 (MQ=255)             ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg                 >  1:225033/1‑36 (MQ=255)             ttCGCTGTTAACGTCATCAGCTCTAATAACGTGAAg                 <  1:749685/36‑1 (MQ=255)                gCTGTTAACGTCATCAGCTCTAATAACGCGAAGTCa              >  1:4626741/1‑36 (MQ=25)                  tGTTAACGTCATCAGCTGTAATAACGTGAAGTCAAt            <  1:6634920/36‑1 (MQ=25)                   gTTAACGTCATCAGCTCTAATAACGTGAAGTCAATa           <  1:4713786/36‑1 (MQ=255)                    ttAACGTCATCAGCTCTAATAACGTGAAGTCAATAg          <  1:1991602/36‑1 (MQ=255)                      aaCGTCATCAGCTCTAATAACGTGAAGTCAATAGcc        <  1:3325816/36‑1 (MQ=255)                      aaCGTCATCAGCTCTAATAACGTGAAGTCAATAGcc        >  1:5780855/1‑36 (MQ=255)                       aCGTCATCAGCTCTAATAACGTGAAGTCAATAGCCt       <  1:386732/36‑1 (MQ=255)                       aCGTCATCAGCTCTAATAACGTGAAGTCAATAGCCt       >  1:1073422/1‑36 (MQ=255)                        cGTCATCAGCTCTAATAACGTGAAGTCAATAGACTg      >  1:3632350/1‑36 (MQ=255)                          tcatcaGCTCTAATAACGTGAAGTCAATAGCCTGGc    >  1:4997744/1‑36 (MQ=255)                          tcatcaGCTCTAATAACGTGAAGTCAATAGCCTGGc    <  1:6160405/36‑1 (MQ=255)                          tcatcaGCTCTAATAACGGTAAGTCAATAGCCTGGc    <  1:7197219/36‑1 (MQ=21)                            atcaGCTCTAATAACGTGAAGTCAATAGCCTGGCGt  <  1:2648148/36‑1 (MQ=255)                            atcaGCTCTAATAACGTGAAGTCAATAGCCTGGCGt  >  1:757064/1‑36 (MQ=255)                                 |                               CTGGCTTCTGCTTTCGCTGTTAACGTCATCAGATCTAATAACGTGAAGTCAATAGCCTGGCGT  >  REL606/1248348‑1248410 Base quality scores:  ATCG  < 3 ≤  ATCG  < 9 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 32 ≤  ATCG