Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL606573,2020T→G100.0% 1.2 8H96P (CAC→CCC) ECB_00530conserved hypothetical protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (1/0):  total (8/0)

TCTGGCGCAGACCGGCACGCCACAGCGGCGGGTGCACCTTCATGCT  >  REL606/573170‑573215
                                |             
tCTGGCGCAGACCGGCACGCCACAGCGGCGGGGGcc            >  1:6266932/1‑35 (MQ=0)
 cTGGCGCAGACCGGCACGCCACAGCGGCGGGGGcgc           >  1:1077817/1‑34 (MQ=1)
  tGGCGCAGACCGGCACGCCACAGCGGCGGGGGcggc          >  1:46277/1‑33 (MQ=1)
     cgcAGACCGGCACGCCACAGCGGCGGGGGCCccccc       >  1:6558675/1‑33 (MQ=1)
      gcAGACCGGCACGCCACAGCGGCGGGTGCACCCTcc      >  1:1812278/1‑35 (MQ=0)
      gcAGACCGGCACGCCACAGCGGCGGGGGGGCCTTcc      >  1:4217677/1‑35 (MQ=1)
          aCCGGCACGCCACAGCGGCGGGGGCAGCTTTAtttt  >  1:4021281/1‑33 (MQ=1)
          aCCGGCACGCCACAGCGCCGGGGGCCCCTCCCTGcc  >  1:419221/1‑35 (MQ=0)
                                |             
TCTGGCGCAGACCGGCACGCCACAGCGGCGGGTGCACCTTCATGCT  >  REL606/573170‑573215

Base quality scores:  ATCG  < 3 ≤  ATCG  < 4 ≤  ATCG  < 7 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG