Read alignment evidence...
  seq id position change freq score reads annotation genes product
*REL6063,671,9740C→A22.2% 17.254C22F (TGC→TTC) yiaIpredicted hydrogenase, 4Fe‑4S ferredoxin‑type component
Reads supporting (aligned to +/- strand):  new base (0/12):  ref base (36/6):  total (36/18)

CAGCGCAATCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTT  >  REL606/3671939‑3672008
                                   |                                  
cAGCGCAATCCTGATTCTCATGATGCGACACTgcgc                                    >  1:5234159/1‑36 (MQ=255)
 aGCGCAATCCTGATTCTCATGATGCGACACTGCGCa                                   >  1:3585583/1‑36 (MQ=255)
 aGCGCAACCCTGATTCTCATGATGCGACACTGCGCa                                   <  1:4802766/36‑1 (MQ=255)
  gcgcAATCCTGATTCTCATGATGCGACACTGCGCaa                                  >  1:4063803/1‑36 (MQ=255)
  acgcAATCCTGATTCTCATGATGCGACACTGCGCaa                                  <  1:1394884/35‑1 (MQ=255)
   cgcAATCCTGATTCTCATGATGCGACACTGCGCAAg                                 <  1:2386816/36‑1 (MQ=255)
     cAATCCTGATTCTCATGATGCGACACTGCGCAAGcc                               >  1:6559431/1‑36 (MQ=255)
     cAACCCTGATTCTCATGATGCGACACTGCGCAAGcc                               <  1:5927193/36‑1 (MQ=255)
      aaTCCTGATTCTCATGATGCGACACTGCGCAAGCCa                              <  1:4149322/36‑1 (MQ=255)
      aaTCCTGATTCTCATGATGCGACACTGCGCAAGCCa                              >  1:4354459/1‑36 (MQ=255)
      aaTCCTGATTCTCATGAGGCGACACGGCGCAAGCCa                              <  1:4380956/36‑1 (MQ=25)
       aTCCTGATTCTCATGATGCGACACTGCGCAAGCCAc                             >  1:7163521/1‑36 (MQ=255)
         cttGATTCTCGAGATGCGACACTGCGAAAGCCACtt                           <  1:4404900/34‑1 (MQ=21)
           tGATTCTCATGATGCGACACTGCGCAAGCCACTTca                         >  1:6584819/1‑36 (MQ=255)
             tttCTCATGATGCGACACGGCGAAAGCCACTTcaca                       <  1:5267797/35‑1 (MQ=25)
             aTTCTCATGATGCGACACCGCGCAAGCCACCTcaca                       >  1:4148492/1‑36 (MQ=25)
             aTTCTCAGGATGCGACACTGCGAAAGCCACTTcaca                       <  1:5903704/36‑1 (MQ=25)
              ttCTCATGATGCGACACTGCGCAAGCCACTTCACAg                      >  1:6396190/1‑36 (MQ=255)
               tctcATGATGCGACACTGCGCAAGCCACTTCACAgg                     >  1:2693427/1‑36 (MQ=255)
               tctcATGAGCCGACACTGCAAAAGCCACTTCACAgg                     <  1:1204864/36‑1 (MQ=17)
                ctcATGATGCGACACTGCGCAAGCCACTTCACAGGt                    >  1:1193583/1‑36 (MQ=255)
                 tcATGATGCGACACTGCGCAAGCCACTTCACAggaa                   >  1:4783156/1‑34 (MQ=255)
                  cATGATGCGACACTGCGCAAGCCACTTCACAGGTAc                  >  1:6849916/1‑36 (MQ=255)
                  cATGATGCGACACTGCGCAAGCCACTTCACAGGTAc                  >  1:1084208/1‑36 (MQ=255)
                  cATGATGCGACACTGCGCAAGCCACTTCACAGATAc                  >  1:4348778/1‑36 (MQ=255)
                  cATGATGCGACACCGCGCAAGCCACTTCACAGGTAc                  >  1:991715/1‑36 (MQ=255)
                  cATGACGCAACACGGCGAAAGCCACTTCACAGGTAc                  <  1:3678272/36‑1 (MQ=18)
                  aatgaggCGACACGGCGAAAGCCACTTCACAGGTAc                  <  1:5482350/35‑1 (MQ=21)
                   atgatgCGACACTGCGCAAGCCACTTCACAGGTACg                 >  1:3471376/1‑36 (MQ=255)
                   atgatgCGACACTGCGCAAGCCACTTCACAGGTACg                 >  1:1201722/1‑36 (MQ=255)
                   atgatgCGACACTGCGCAAGCCACTTCACAGGTACg                 >  1:6388318/1‑36 (MQ=255)
                   atgatgCGACACTGCGCAAGCCACTTCACAGGTACg                 >  1:5383398/1‑36 (MQ=255)
                    tgatgCGACACTGCGCAAGCCACTTCACAGGTACgg                >  1:87560/1‑36 (MQ=255)
                     gatgCGACACTGCAAAAGCCACTTCACAGGTACGGc               <  1:7447231/36‑1 (MQ=21)
                     gatgCCACACTGCAAAAGCCACTTCACAGGTACGGc               <  1:7441778/36‑1 (MQ=18)
                      atgCGACACTGCGCAAGCCACTTCCCAGGTACGGCa              >  1:3174289/1‑36 (MQ=255)
                      atgCGACACTGCGCAAGCCACTTCACAGGTACGGCa              >  1:2766088/1‑36 (MQ=255)
                      atgCGACACTGCGCAAGCCACTTCACAGGTACGGCa              >  1:5340458/1‑36 (MQ=255)
                      atgCGACACTGCGCAAGCCACTTCACACCTACCGCa              >  1:2591607/1‑36 (MQ=21)
                       tgCGACACTGCGCAAGCCACTTCACAGGTACGGCaa             >  1:4195189/1‑36 (MQ=255)
                       tgCGACACTGCGCAAGCCACATCACAGGTACGGCaa             >  1:578795/1‑36 (MQ=255)
                       tgCAACACTGCAAAAGCCACTTCACAGGTACGGCag             <  1:2070786/36‑2 (MQ=18)
                         cGACAGGGCGAAAGCCACTTCACAGGTACGGCAAcc           <  1:6414694/36‑1 (MQ=17)
                         cGACACTGCGCAAGCCACTTCACAGGTACGGCCAcc           >  1:4180906/1‑36 (MQ=255)
                         acacagTGCGAAAGCCACTTCACAGGTACGGCAAcc           <  1:235860/34‑1 (MQ=17)
                          gACACTGCGCAAGCCACTTCACAGGTACGGCAACCg          >  1:5097328/1‑36 (MQ=255)
                           acacTGCGCAAGCCACTTCACAGGTACGGCAACCGa         >  1:3993365/1‑36 (MQ=255)
                           acacGGCGAAAGCCATTTCACAGGTACGGCAACCGa         <  1:6751239/36‑1 (MQ=18)
                             acTGCGCAAGCCACTTCACAGGTACGGCAACCGATa       >  1:3128905/1‑36 (MQ=255)
                             acTGCGCAAGCCACTTCACAGGTACGGCAACCGATa       >  1:1668662/1‑36 (MQ=255)
                             acTGCGCAAGCCACTTCACAGGTACGGCAACCGATa       >  1:5234459/1‑36 (MQ=255)
                               tGCGCAAGCCACTTCACAGGTACGGCAACCGATACa     >  1:1101888/1‑36 (MQ=255)
                               tGCGCAAGCCACTTCACAGGTACGGCAACCGATACa     >  1:7434028/1‑36 (MQ=255)
                               tGCGCAAGCCACTTCACAGGTACGGCAACCGATACa     >  1:3290029/1‑36 (MQ=255)
                               tGCGCAAGCCACTTCACAGGTACGGCAACCGAAACa     >  1:4237698/1‑36 (MQ=255)
                                gcgcAAGCCACTTCACAGGTACGGCAACCGATACAt    >  1:60240/1‑36 (MQ=255)
                                  gcAAGCCACTTCACAGGTACGGCAACCGATACAttt  >  1:5150168/1‑36 (MQ=255)
                                   |                                  
CAGCGCAATCCTGATTCTCATGATGCGACACTGCGCAAGCCACTTCACAGGTACGGCAACCGATACATTT  >  REL606/3671939‑3672008

Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 15 ≤  ATCG  < 27 ≤  ATCG  < 32 ≤  ATCG