Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	REL606	2,433,454	0	A→T	18.6%	11.3	59	M187F (ATT→TTT)	ypdA	predicted sensory kinase in two‑component system with YpdB
Reads supporting (aligned to +/- strand):  new base (11/0):  ref base (21/27):  total (32/27)

CCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAA  >  REL606/2433421‑2433489                                  |                                    ccAATGATCCTCGGTAGCGTCTGTATCGGCTTTTtt                                   >  1:4041235/1‑36 (MQ=255)  cAATGATCCTCGGTAGCGTCTGTATCGGCTTTTttt                                  >  1:2468860/1‑35 (MQ=255)  cAATGATCCTCGGTAGCGTCTGTATCGGCTTTATtg                                  <  1:620975/36‑1 (MQ=255)  cAATGATCCTCGGTAGCGTCTGTATCGGCCTTTttt                                  >  1:5510740/1‑35 (MQ=25)   aaTGATCCTCGGTAGCGTCTGTATCGGCTTTTtttt                                 >  1:1857638/1‑34 (MQ=37)   aaTGATCCTCGGTAGCGTCTGTATCGGCCTTTtttt                                 >  1:1955956/1‑34 (MQ=25)    aTGATCCTCGGTAGCGTCTGTATCGGGTTTTttttt                                >  1:820145/1‑33 (MQ=21)        tCCTCGGTAGCGTCTGTATCGGCTTTTTTTTTCttt                            >  1:1603237/1‑35 (MQ=21)          cTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTg                          <  1:5119623/36‑1 (MQ=255)           tctgTAGCGTCTGTATCGGCTTTATTGTGCTTCTgg                         <  1:2874595/33‑1 (MQ=255)           tCGGTAGCGTCTGTAGCGGCTTTAGTGTGCTTCTgg                         <  1:6451144/36‑1 (MQ=25)            cGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGt                        <  1:6940372/36‑1 (MQ=255)             ggTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTg                       <  1:307187/36‑1 (MQ=255)              gTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGc                      <  1:6897710/36‑1 (MQ=255)               tAGGGTCTGTATCGGCTTTATTGTGCTTCTGGTGCa                     <  1:4349469/36‑1 (MQ=255)                 gCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCaaa                   <  1:2454441/36‑1 (MQ=255)                 gCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCaaa                   <  1:6259485/36‑1 (MQ=255)                 gCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCaaa                   <  1:1607938/36‑1 (MQ=255)                 gCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCaaa                   <  1:3416557/36‑1 (MQ=255)                  cGTCTGTATCGGCTTTTTTTTGCTTCTGGTGTAAAg                  >  1:2031881/1‑36 (MQ=18)                  cGTCTGTATCGGCTTTTTTTTGCTTCTGGTGCCAAg                  >  1:4926904/1‑36 (MQ=18)                  cGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAg                  <  1:5695003/36‑1 (MQ=255)                  cGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAg                  <  1:1991377/36‑1 (MQ=255)                  cGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAg                  <  1:2509114/36‑1 (MQ=255)                  cGTCTGTATCGGCTTTATTGTGCTTCCGGTGCAAAg                  >  1:1705087/1‑36 (MQ=255)                   gTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGc                 <  1:1050565/36‑1 (MQ=255)                    tCTGTATCGGCTTTATTGTGCTTTTTGTGTAAAGCg                >  1:4509465/1‑36 (MQ=21)                     cTGTATCGGCTTTTTTTTTCTTTTTGTTCAAAACGt               >  1:1600795/1‑36 (MQ=0)                      tGTATCGGCTTTTTTTTGCTTCTGGTGCAAAGGGtt              >  1:2739022/1‑36 (MQ=18)                       gTATCGGCTTTATTGTGCTTTTGGTGCAAAGCGTTg             >  1:4866711/1‑36 (MQ=255)                       gTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTg             <  1:4155799/36‑1 (MQ=255)                        tATCGGCTTTATTTTGCTTCTGGTGCAAAGCGCTGa            >  1:5601275/1‑36 (MQ=21)                        tATCGGCTTTATTGTGCTTCTGGTGCAACGCGCTGa            >  1:4595458/1‑36 (MQ=25)                        tATCGGCTTTATTGTGCTTCTGGTGCAAAGCGttta            >  1:2103974/1‑34 (MQ=255)                        tATCGGCTTTATTGTGCTTCCGGTGCAAAACGTTGa            >  1:6967428/1‑36 (MQ=21)                         aTCGGCTTTATTGTGCTTCTTGTGCAAAGCGTTTAg           >  1:1896261/1‑36 (MQ=25)                         aTCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAg           <  1:783526/36‑1 (MQ=255)                          tCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAgg          >  1:5660974/1‑36 (MQ=255)                          tCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAgg          <  1:5870622/36‑1 (MQ=255)                          gctgCTTNATTGTGCTTCTGGTGCAAAGCGTTGAgg          <  1:613390/33‑1 (MQ=255)                           cGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAggg         >  1:3301782/1‑36 (MQ=255)                           cGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAggg         <  1:1012178/36‑1 (MQ=255)                            gtcTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        <  1:1870379/34‑1 (MQ=255)                            ggCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        <  1:5021093/36‑1 (MQ=255)                            ggCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        >  1:4760471/1‑36 (MQ=255)                            ggCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        >  1:3504965/1‑36 (MQ=255)                            ggCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        >  1:6676460/1‑36 (MQ=255)                            ggCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        >  1:5914714/1‑36 (MQ=255)                            ggCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGc        >  1:1163649/1‑36 (MQ=255)                             gCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCg       >  1:235648/1‑36 (MQ=255)                             gCTTTATTGTGCTTCTGGTGAAAAGGGTTGAGGGCg       >  1:7030020/1‑36 (MQ=25)                               tttATTGTGCTTCTGGTGCAAAGCGTTGAGGGCgta     >  1:663924/1‑34 (MQ=255)                               tttATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaa     <  1:2824136/36‑1 (MQ=255)                               tttATTGTGCTTCTGGTGCAAAGCGTTGACAGCGaa     >  1:4484023/1‑36 (MQ=25)                                ttATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaa    <  1:552129/36‑1 (MQ=255)                                ttATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaa    >  1:2475775/1‑36 (MQ=255)                                 tATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaaa   >  1:6831656/1‑36 (MQ=255)                                 tATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaaa   <  1:5790958/36‑1 (MQ=255)                                 tATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaaa   <  1:2599638/36‑1 (MQ=255)                                  aTTGTGCTTCTGGTGCAAAGCGTTGAGGGCGtaatt  >  1:967124/1‑34 (MQ=25)                                  aTTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaaaa  >  1:5901480/1‑36 (MQ=255)                                  aTTGTGCTTCTGGTGCAAAGCGTTGAGGGCGaaaaa  <  1:3171581/36‑1 (MQ=255)                                  |                                    CCAATGATCCTCGGTAGCGTCTGTATCGGCTTTATTGTGCTTCTGGTGCAAAGCGTTGAGGGCGAAAAA  >  REL606/2433421‑2433489 Base quality scores:  ATCG  < 3 ≤  ATCG  < 7 ≤  ATCG  < 14 ≤  ATCG  < 26 ≤  ATCG  < 32 ≤  ATCG